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Plasmid#128655Purposemammalian expression of Flag-Polß(V306R) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationV306RPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(L301R/V306R)-IRES-puro
Plasmid#128656Purposemammalian expression of Flag-Polß(L301R/V306R) with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationL301R/V306RPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(KTM)-IRES-puro
Plasmid#128672Purposemammalian expression of Flag-Polß(KTM: K41R/K61R/K81R) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationK41R/K61R/K81RPromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(K206A/K244A)-IRES-puro
Plasmid#128683Purposemammalian expression of Flag-Polß(K206A/K244A) protein with puromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationK206A/K244APromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-NCOA7g3 (BB24)
Plasmid#139459PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKLV-gRNA-Bcan-Ntrk1_4
Plasmid#136413PurposeLentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP.DepositorInsertU6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKLV-gRNA-Myb-Qk_1
Plasmid#136415PurposeLentiviral expression of gRNAs targeting intron 4 of murine Qk and intron 9 of murine Myb. Also constitutively expresses Puromycin linked to TagBFP.DepositorInsertU6_sgRNA(Myb)_U6_sgRNA(Qk)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-TIRAP-cGASΔN-HA
Plasmid#130921PurposeExpresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection markerDepositorInsertTIRAP-ΔNcGAS
UseTagsHAExpressionMammalianMutationcGAS N terminus (aa1-159) replaced with the N ter…PromoterCMVAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Fyn-cGASΔN-HA
Plasmid#130922PurposeExpresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the Fyn dual acylation motif (aa1-16); Puromycin selection markerDepositorInsertFyn-ΔNcGAS
UseTagsHAExpressionMammalianMutationcGAS N terminus (aa1-159) replaced with the dual …PromoterCMVAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL405 CHYRON1 integration construct
Plasmid#126442PurposeTo integrate the CHYRON1 locus at HEK293site3.DepositorInsertspU6-CHYRON1 hgRNA
pCMV-puro
UseTagsExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6Available sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-HA-MEK5 CBM-E
Plasmid#53199PurposeExpresses HA tagged human MEK5 L280A/H281A/M323A/Q332A from puromycin resistance retroviral vectorDepositorInsertMEK5 (MAP2K5 Human)
UseRetroviralTagsHAExpressionMammalianMutationL280A/H281A/M323A/Q332APromoterAvailable sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-ADAMTS18 (P1035S)
Plasmid#29558DepositorInsertADAMTS18 (ADAMTS18 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationchanged proline 1035 to serinePromoterAvailable sinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-ADAM29 (S112F)
Plasmid#31145DepositorInsertADAM29 (ADAM29 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationSerine 112 changed to PheylalninePromoterAvailable sinceSept. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-ADAM29 (E111K)
Plasmid#31144DepositorInsertADAM29 (ADAM29 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationGlutamic acid 111 changed to LysinePromoterAvailable sinceSept. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-ADAMTS18 (Q1002X)
Plasmid#29557DepositorInsertADAMTS18 (ADAMTS18 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationchanged glutamine 1002 to stop codonPromoterAvailable sinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-ADAMTS18 (Q904X)
Plasmid#29556DepositorInsertADAMTS18 (ADAMTS18 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationchanged glutamine 904 to stop codonPromoterAvailable sinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-ADAMTS18 (C638S)
Plasmid#29555DepositorInsertADAMTS18 (ADAMTS18 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationchanged cysteine 638 to serinePromoterAvailable sinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-ADAMTS18 (G312E)
Plasmid#29553DepositorInsertADAMTS18 (ADAMTS18 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationchanged glycine 312 to glutamic acidPromoterAvailable sinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
PB-CMV-MCS-Puro
Plasmid#219794PurposeMammalian expression plasmids using piggyBac Transposon SystemDepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgRNA
Plasmid#71409PurposeThere is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBIDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only