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loxP-myctagBFP-STOP-loxP-mCherry-NTR

Plasmid

#108456

Purpose

This plasmid can be used to recombine the loxP-myctagBFP-STOP-loxP-mCherry-NTR cassette (or only mCherry-NTR) into a BAC

Depositor

Nadia Mercader Huber

Article

Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

Insert

loxP-myctagBFP-STOP-loxP-mCherry-NTR

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO Myc ArC Long WT

Plasmid

#59809

Purpose

Allows the integration of Myc ArC Long in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora C (AURKC Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pGW1-Myc-DJ1-L166P/K130R

Plasmid

#29349

Depositor

Mark Cookson

Article

Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

Insert

DJ1 (PARK7 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Leu166Pro and Lys130Arg

Promoter

Available since

April 1, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5 FRT TO Myc ArC K72R

Plasmid

#59811

Purpose

Allows the integration of Myc ArC K72R in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora C (AURKC Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

K72R, kinase mutant

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5 Myc-tagged PSK (K57A)

Plasmid

#197150

Purpose

Expression of kinase-deficient MYC-tagged PSK1-alpha (K57A) / TAOK2 (K57A) in mammalian cells

Depositor

Jonathan Morris

Article

Moore et al J Biol Chem. 2000 Feb 11;275(6):4311-22.

Insert

TAOK2 (TAOK2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Changed K 57 to A

Promoter

SV40

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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MYC-NKX3.1

Plasmid

#42895

Depositor

Edward Gelmann

Article

Markowski et al Cancer Res. 2008 Sep 1;68(17):6896-901.

Insert

NKX3.1 (NKX3-1 Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA-IgKappa-myc-dL5- 2XG4S-GPI

Plasmid

#153308

Purpose

Expresses GPI-anchored dL5** in mammalian cells. (FAP-GPI)

Depositor

Marcel Bruchez

Article

Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

Insert

IgKappa-myc-dL5-2XG4S-GPI

Use

Tags

myc-dL5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fSNAP-Phleomycin

Plasmid

#188915

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-Phleomycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fSNAP-Hygromycin

Plasmid

#188914

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-Hygromycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-HALO-Hygromycin

Plasmid

#188929

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-Hygromycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-HALO-Kanamycin

Plasmid

#188933

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-Kanamycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-HALO-Phleomycin

Plasmid

#188930

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-Phleomycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-Phleomycin

Plasmid

#188925

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-Phleomycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-Hygromycin

Plasmid

#188924

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-Hygromycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-Phleomycin

Plasmid

#188920

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-Phleomycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-Hygromycin

Plasmid

#188919

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-Hygromycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFH2.98_PB-EPN1-MCP_Myc-T2A-eGFP-PGK-Blast

Plasmid

#205543

Purpose

Express EPN1-MCP-Myc-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN1-MCP-Myc

Use

Piggybac

Tags

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-MYC(Δ1-143)-GFP-IRES-mCherry (ΔTAD)

Plasmid

#231233

Purpose

MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) for transient expression in mammalian cells

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

MYC (MYC Human)

Use

Tags

Expression

Mammalian

Mutation

TAD deleted (delta1-143); numbering based on sequ…

Promoter

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-cmyc

Plasmid

#16011

Purpose

mammalian expression of human cmyc

Depositor

Wafik El-Deiry

Article

Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55

Insert

cmyc (MYC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HLA-cmyc-optiT1R3 a21-852

Plasmid

#113949

Purpose

mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R3 (TAS1R3 Human)

Use

Tags

Signal/leader sequence from HLA class I histocomp…

Expression

Mammalian

Mutation

residues 21-852

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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