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pBBK28 pHomL-TDH3p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179012

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pHPyV7-713a

Plasmid

#24728

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

Full genome of Human polyomavirus 7

Use

Viral clone

Tags

Expression

Mutation

Promoter

Available since

June 8, 2010

Availability

Academic Institutions and Nonprofits only

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pHPyV6-607a

Plasmid

#24727

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

Full genome of Human polyomavirus 6

Use

Viral clone

Tags

Expression

Mutation

Promoter

Available since

June 8, 2010

Availability

Academic Institutions and Nonprofits only

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pBABE-neo

Plasmid

#1767

Purpose

Retroviral vector for cloning and expressing your gene of interest. Neomycin selection.

Depositor

Bob Weinberg, Jay Morgenstern, Hartmut Land

Article

Morgenstern et al Nucleic Acids Res. 1990 Jun 25;18(12):35

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2005

Availability

Academic Institutions and Nonprofits only

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pEE515

Plasmid

#149288

Purpose

T-DNA encoding TRV2 complete genome with MCS

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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PECAM-eGFP

Plasmid

#14688

Depositor

Victoria Bautch

Article

Kearney et al Blood. 2004 Jun 15. 103(12):4527-35.

Insert

PECAM promoter/enhancer (Pecam1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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nls-Flamindo2

Plasmid

#73939

Purpose

Expresses biosensor for cAMP in mammalian cells with nuclear localization signal

Depositor

Tetsuya Kitaguchi

Article

Odaka et al PLoS One. 2014 Jun 24;9(6):e100252. doi:

Insert

nls-Flamindo2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pET24_NusA-Tus

Plasmid

#165959

Purpose

Expression of soluble E. coli Tus protein

Depositor

Keith Pardee

Article

Norouzi et al ACS Synth Biol. 2021 Jun 23. doi: 10.102

Insert

Tus

Use

Tags

His-NusA-Tev

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pX330S-3

Plasmid

#58779

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAG504-frFAST

Plasmid

#160055

Purpose

Expresses frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

frFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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V96 ELP

Plasmid

#68392

Purpose

Elastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residue

Depositor

Andrew MacKay

Article

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

V96 ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pEC3135 (pERASE)

Plasmid

#220306

Purpose

Enables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS*

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-APEX2-NIK3x

Plasmid

#129274

Purpose

Expression of APEX2 in the nucleolus

Depositor

Alice Ting

Article

Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

Insert

GFP-APEX2-NIK3x

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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S96 ELP

Plasmid

#68393

Purpose

Elastin-like polypeptide (VPGxG) with 96 repeats of Serine guest residue

Depositor

Andrew MacKay

Article

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

S96 ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pGL2-2xCLEAR-luciferase

Plasmid

#81120

Purpose

Reporter activity of the CLEAR network

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

2xCLEAR

Use

Luciferase

Tags

luciferase

Expression

Mutation

Promoter

2xCLEAR

Available since

Dec. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pX330 spCas9-mSA

Plasmid

#113096

Purpose

pX330 vector carrying spCas9-mSA for gene editing in mammalian cell lines

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

CAS9-MSA

Use

Synthetic Biology

Tags

MSA (monomeric streptavidin

Expression

Mutation

none

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Wnk1

Plasmid

#38780

Depositor

Melanie Cobb

Article

Xu et al J Biol Chem. 2000 Jun 2;275(22):16795-80

Insert

Wnk1 (Wnk1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pcNB1

Plasmid

#89241

Purpose

Resurfaced Cell-penetrating GFP nanobody

Depositor

Brian McNaughton

Article

Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

Insert

pcNB1 (GFP)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-A20

Plasmid

#22141

Depositor

Yihong Ye

Article

Li et al Biochim Biophys Acta. 2008 Jun . 1783(6)

Insert

A20 (TNFAIP3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
2XMyc-LRRK2-WT

Plasmid

#25361

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Tags

2XMyc

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC18-mini-Tn7T-Gm-lux

Plasmid

#64963

Purpose

mini-Tn7 luxCDABE transcriptional fusion vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

lux operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSIM24-GUS

Plasmid

#67882

Purpose

Plant Gene Expression Vector pSIM24 containing the GUS (Beta-glucuronidase) reporter gene

Depositor

Indu Maiti

Article

Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

Insert

uidA

Use

Tags

Expression

Plant

Mutation

T120A

Promoter

M24

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pTOX6

Plasmid

#127451

Purpose

Encodes Tse2 toxin and amilCP chromoprotein. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

tse2 toxin
amilCP chromoprotein

Use

Tags

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

pRha and tac

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TLS 2: hTLS.pCDNA1

Plasmid

#21828

Depositor

David Ron

Article

Crozat et al Nature. 1993 Jun 17;363(6430):640-4.

Insert

TLS (FUS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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mApple-N1

Plasmid

#54567

Purpose

Localization: N1 Cloning Vector, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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TRE-mCherry

Plasmid

#92202

Purpose

Fluorescent protein reporter construct

Depositor

Alice Ting

Article

Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

mCherry

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

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piGECInano-N1

Plasmid

#186190

Purpose

Expression of small FRET-based near-infrared genetically-encoded calcium indicator in mammalian cells

Depositor

Vladislav Verkhusha

Article

Matlashov et al Front Cell Dev Biol. 2022 Jun 29;10:8801

Insert

iGECInano

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-GFP-Sec61β

Plasmid

#186597

Purpose

Third generation lentiviral vector expressing GFP-Sec61β

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Sec61β (SEC61B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGCDNsam-Hnf4a-IRES-GFP

Plasmid

#33006

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

hepatic nuclear factor 4 alpha (Hnf4a Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
V5-APEX2-LMNA

Plasmid

#129275

Purpose

Expression of APEX2 at nuclear lamina

Depositor

Alice Ting

Article

Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

Insert

V5-APEX2-LMNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-myc-Wnk1

Plasmid

#38779

Depositor

Melanie Cobb

Article

Xu et al J Biol Chem. 2000 Jun 2;275(22):16795-80

Insert

Wnk1 (Wnk1 Rat)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 20, 2013

Availability

Academic Institutions and Nonprofits only
Filamin-C-stFRET

Plasmid

#61107

Purpose

Expression of Filamin with C-term stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

filamin-stFRET

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
EasyFusion T2A-H2B-TagBFP

Plasmid

#113086

Purpose

General donor vector with T2A-H2B-TagBFP coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-TagBFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15-UbE2D1

Plasmid

#15782

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)

Use

Tags

6x His, T7 tag, and Xpress tag (enterokinase clea…

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
ATM N-terminal sgRNA

Plasmid

#207089

Purpose

pX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ATCATTAAGTACTAGACTCA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGM24951

Plasmid

#169088

Purpose

MoClo level 0 Tomato E8 promoter module

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

Tomato E8 promoter

Use

Synthetic Biology; Moclo cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-BAF

Plasmid

#172447

Purpose

Expression of nuclear protein BAF with an N-terminal mCherry tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

BAF

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVT36b

Plasmid

#184437

Purpose

CRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffold

Depositor

Huimin Zhao

Article

Tran et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-IRAlus-Nicn1

Plasmid

#92345

Purpose

Plasmid for expression of GFP gene with a Nicn1 gene derived IR Alu element containing 3'UTR.

Depositor

Ling-Ling Chen, Gordon Carmichael

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Nicn1 gene derived IR Alu element containing 3'UTR (NICN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-NLRP10

Plasmid

#38141

Depositor

Thomas Kufer

Article

Lautz et al Cell Microbiol. 2012 Jun 4. doi: 10.1111

Insert

NLRP10 (NLRP10 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only
pTOX8

Plasmid

#127453

Purpose

Encodes MqsR toxin and tsPurple chromoprotein. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

mqsR toxin
tsPurple chromoprotein

Use

Tags

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

apFAB46 and pRha

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
EasyFusion T2A-H2B-GFP

Plasmid

#112851

Purpose

General donor vector with T2A-H2B-GFP coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-GFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

No

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGCDNsam-Foxa1-IRES-GFP

Plasmid

#33007

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

forkhead box A1 (Foxa1 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pKanCMV_mRuby3

Plasmid

#172854

Purpose

mRuby3 expressed under the CMV promotor

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

mRuby3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Paxillin-pEGFP

Plasmid

#15233

Depositor

Rick Horwitz

Article

Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

Insert

paxillin (PXN Chicken)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
V5-APEX2-SENP2

Plasmid

#129276

Purpose

Expression of APEX2 at nuclear pore

Depositor

Alice Ting

Article

Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

Insert

V5-APEX2-SENP2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSNAP-H3 Zeo

Plasmid

#132427

Purpose

Expressing SNAP-tagged histone H3.1 in mammalian cells

Depositor

John M. Greally

Article

Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

Insert

Histone H3.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
V48 ELP

Plasmid

#68395

Purpose

Elastin-like polypeptide (VPGxG) with 48 repeats of valine guest residue

Depositor

Andrew MacKay

Article

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

V48 ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGCDNsam-Foxa2-IRES-GFP

Plasmid

#33008

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

forkhead box A2 (Foxa2 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag-Smad1

Plasmid

#14044

Depositor

Joan Massague

Article

Liu et al Nature. 1996 Jun 13. 381(6583):620-3.

Insert

Smad1 (SMAD1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge

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