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Plasmid#122610Purposeexpress evoFERNY-BE4max in mammalian cellsDepositorInsertevoFERNY-BE4max
UseTagsExpressionMammalianMutationH102P D104NPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-Sfp R4-4 mutant
Plasmid#173491PurposeExpression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.DepositorInsertSfp-R44
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_EF1a-->Gss52SR4-LaM4-P2A-NLSIFP
Plasmid#162229PurposeLentiviral vector for the expression of Gss52SR4-LaM4-P2A-NLSIFP in mammalian cellsDepositorInsertLaG17 GFP nanobody SynNotch tTA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_220
Plasmid#91113PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: noneDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
paavCAG-pre-mGRASP-2A-dTomato
Plasmid#51902Purposepre-2AtoDepositorInsertpresynaptic mGRASP
UseAAV and Cre/LoxTagsExpressionMutationPromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAGM24951
Plasmid#169088PurposeMoClo level 0 Tomato E8 promoter moduleDepositorInsertTomato E8 promoter
UseSynthetic Biology; Moclo cloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_210
Plasmid#91108PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: noneDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKHH030
Plasmid#89358PurposehU6 sgRNA cloning vector for CDKO systemDepositorInsertGFP, Puromycin resistance
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002
Plasmid#102827PurposeBacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteinsDepositorInsertSpyCatcher002
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
HIV-1 integrase F185K/C280S in pET-15b
Plasmid#61669PurposeHIV-1 integrase F185K/C280S in pET-15bDepositorInsertHIV-1 integrase F185K/C280S
UseSynthetic BiologyTagsHisExpressionMutationPromoterT7 promotorAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135B2.0
Plasmid#167159PurposeGolden Gate entry vector to express the 5th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
peGFP-N1-SHB
Plasmid#139605PurposeExpresses eGFP-SHB fusion protein in mammalian cellsDepositorInsertSHB (SHB Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LSSmCherry1
Plasmid#89986PurposeLong stokes-shift variant of red fluorescent protein mCherryDepositorInsertLSSmCherry1
UseTags6xHis-T7-XpressExpressionBacterialMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBad-mKalama1
Plasmid#14892PurposeBacterial expression of mKalama1 blue fluorescent proteinDepositorInsertmKalama1
UseTagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…PromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNO183
Plasmid#101068PurposeppsR2-bphP1 bicistronic operon plasmidDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationinsert guanine at position 430 to fix frameshift …PromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136B2.0
Plasmid#167160PurposeGolden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-pMJ915v2
Plasmid#88916PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKM444
Plasmid#108319PurposeFor performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage IntegraseDepositorInsertsChe9C RecT
Bxb1 Integrase
Tet Repressor
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
ykgMO-IGS-sfGFP
Plasmid#207231PurposeCalprotectin sensing promoter with sfGFP reporterDepositorInsertykgMO-IGS
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pS3
Plasmid#50596PurposeBackbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5DepositorInsertsaroE
aroD
aroB
aroG*
ppsA
tktA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only