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pUC57 mini-ZsGreen-P2A

Plasmid

#222648

Purpose

Vector for cloning gene of interest for in vitro transcription

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

ZsGreen

Use

In vitro transcription

Tags

Expression

Mutation

mutated stop codon prior to a P2A peptide

Promoter

T7

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_SRR124.5_tagBFP

Plasmid

#163753

Purpose

gRNA expression vector containing the tagBFP fluorescent marker to target the region upstream of the SRR124 SOX2 enhancer in human cells.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

gRNA_SRR124.5

Use

CRISPR

Tags

tagBFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_SRR134.3_miRFP670

Plasmid

#163754

Purpose

gRNA expression vector containing the miRFP670 fluorescent marker to target the region downstream of the SRR134 SOX2 enhancer in human cells.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

gRNA_SRR134.3

Use

CRISPR

Tags

miRFP670

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLXB3o1_hygR-MAR10-New BeYDV geminino-SP-nano72VHH-IgG1-MAR10 (GB4954)

Plasmid

#225441

Purpose

Module for stable transformation of Geminino 1.0-n72_h_HC flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.

Depositor

Diego Orzaez

Insert

hygR:MAR10:New BeYDV geminino:SP-nano72VHH-IgG1:MAR10

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF-coSnaA-TEV-His_His-TEV-SnaC

Plasmid

#225059

Purpose

recombinant expression of SnaA in E. coli

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

SnaA

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ar1

Plasmid

#225219

Purpose

Expression androgen receptor 1 in mammalian cells

Depositor

Deshou Wang

Article

Xiao et al Commun Biol. 2024 Sep 16;7(1):1154. doi:

Insert

androgen receptor 1

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ar2

Plasmid

#225220

Purpose

Expression androgen receptor 2 in mammalian cells

Depositor

Deshou Wang

Article

Xiao et al Commun Biol. 2024 Sep 16;7(1):1154. doi:

Insert

androgen receptor 2

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDLterm

Plasmid

#211903

Purpose

Base plasmid for dual-luciferase constructs to measure terminator strength

Depositor

Stan Fields

Article

Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

Type

Empty backbone

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

35S enhancer + 35S minimal promoter

Available since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-SMN2-inclusion-GLuc

Plasmid

#218671

Purpose

Alternative splicing signaling: signal increased with Ex7 inclusion increasing

Depositor

Jingxin Wang

Article

Tang et al Chembiochem. 2022 May 4;23(9):e202200012

Insert

SMN2 (SMN2 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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TCT-Universal plasmid

Plasmid

#221852

Purpose

A universal plasmid for expressing any protein with TetraCysteine motif

Depositor

Artur Krężel

Article

Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

Insert

Tetra Cysteine tag

Use

Tags

Expression

Bacterial

Mutation

No

Promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-ScChd1[Y137A]

Plasmid

#221198

Purpose

ScChd1-aa118-1274 chromatin remodeler with Y137A mutation

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa118-1274)

Use

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

Y137A

Promoter

T7

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL7044

Plasmid

#167215

Purpose

overexpression of PbrR in E. coli for cell-free lead sensing

Depositor

Julius Lucks

Article

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

PbrR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL7061

Plasmid

#167230

Purpose

cell-free cascaded lead sensor

Depositor

Julius Lucks

Article

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pPbr-AKSIRV

Use

Synthetic Biology

Tags

6X His

Expression

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL7079

Plasmid

#167244

Purpose

cell-free lac sensor with GFP output

Depositor

Julius Lucks

Article

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pLac-sfGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL7080

Plasmid

#167245

Purpose

cell-free lac sensor with RFP output

Depositor

Julius Lucks

Article

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pLac-mRFP1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL7081

Plasmid

#167246

Purpose

cell-free lac sensor with Mango output

Depositor

Julius Lucks

Article

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pLac-Mango

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL7082

Plasmid

#167247

Purpose

cell-free lac sensor with XylE output

Depositor

Julius Lucks

Article

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pLac-XylE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL7084

Plasmid

#167248

Purpose

overexpression of LacI

Depositor

Julius Lucks

Article

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pT7-LacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pET3a-XlH3[ChEx]

Plasmid

#221200

Purpose

Xenopus laevis histone H3 with Nterminal tail swapped for ChEx domain of ScChd1.

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

Xl-H3[ChEx]

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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BS-ZJLEU-PTEF1-GFP

Plasmid

#207018

Purpose

Over-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

pTEF1

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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