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Showing: 4271 - 4280 of 151832 results

  2. pAAV-hSyn-hM3D(Gq)-mCherry

    Plasmid
    #50474
    Purpose
    Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM3D(Gq)-mCherry (CHRM3 Human)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50474-plasmid-map-sequence-id-171071

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  7. pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

    Plasmid
    #185917
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8.20m-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185917-plasmid-map-sequence-id-367357

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  9. pVITRO1-dV-IgG1/λ

    Plasmid
    #52214
    Purpose
    Human immunoglobulin gamma 1 antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    Human immunoglobulin gamma 1 constant region
    Human immunoglobulin lambda constant region
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deleted Variable region
    Promoter
    Mouse Elongation Factor 1 Alpha and Rat Elongatio…
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    52214-plasmid-map-sequence-id-432867

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  10. pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)

    Plasmid
    #140005
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140005-plasmid-map-sequence-id-275488

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Showing: 4271 - 4280 of 151832 results