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Plasmid#202476PurposeExpresses the nanobody E7 BoNT-A after phosphate depletionDepositorInsertnanobody E7 BoNT/A
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-JM3
Plasmid#202477PurposeExpresses the nanobody JM3 after phosphate depletionDepositorInsertnanobody JM3
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-J3
Plasmid#202478PurposeExpresses the nanobody J3 after phosphate depletionDepositorInsertnanobody J3
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-m36.4
Plasmid#202479PurposeExpresses the nanobody m36.4 after phosphate depletionDepositorInsertnanobody m36.4
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-Re5D06
Plasmid#202480PurposeExpresses the nanobody Re5D06 after phosphate depletionDepositorInsertnanobody Re5D06
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-Re9F06
Plasmid#202481PurposeExpresses the nanobody Re9F06 after phosphate depletionDepositorInsertnanobody Re9F06
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-ppiB
Plasmid#202482PurposeExpresses peptidyl-prolyl cis-trans isomerase (ppiB) after phosphae depletionDepositorInsertpeptidyl-prolyl cis-trans isomerase (ppiB)
UseTagsExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-LCAmp-trxB
Plasmid#202465PurposeExpresses a guide RNA to silence trxB after phosphate depletionDepositorInserttrxB guide RNA
UseTagsExpressionBacterialMutationPromoterugpBAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-FLuc0-GFPOPT
Plasmid#210594PurposeExpresses SM(FLAG)-nonoptimal Firefly luciferase-optimal GFPDepositorInsertSpaghetti monster (FLAG)-nonoptimal FLuc-Optimal GFP
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-Fluc100-GFPOPT
Plasmid#210596PurposeExpresses SM(FLAG)-optimal Firefly luciferase-optimal GFPDepositorInsertSpaghetti monster (FLAG)-optimal FLuc-Optimal GFP
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SMFLuc0---MS2
Plasmid#210568PurposeExpresses SM(FLAG)-nonoptimal Firefly with 3'UTR MS2 hairpinsDepositorInsertSpaghetti monster (FLAG)-nonoptimal FLuc
UseLentiviral and LuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SMFLuc100---MS2
Plasmid#210570PurposeExpresses SM(FLAG)-optimal Firefly with 3'UTR MS2 hairpinsDepositorInsertSpaghetti monster (FLAG)-optimal FLuc
UseLentiviral and LuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-RLuc0---MS2
Plasmid#210572PurposeExpresses SM(FLAG)-nonoptimal Renilla with 3'UTR MS2 hairpinsDepositorInsertSpaghetti monster (FLAG)-nonoptimal Renilla luciferase
UseLentiviral and LuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-RLuc100---MS2
Plasmid#210573PurposeExpresses SM(FLAG)-optimal Renilla with 3'UTR MS2 hairpinsDepositorInsertSpaghetti monster (FLAG)-optimal Renilla luciferase
UseLentiviral and LuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SM-FLuc0-malat1
Plasmid#210580PurposeExpresses SM(FLAG)-nonoptimal Firefly - 3'UTR malat1 triple helixDepositorInsertSpaghetti monster (FLAG)-nonoptimal FLuc - 3'UTR malat1 triple helix
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SM-FLuc100-malat1
Plasmid#210581PurposeExpresses SM(FLAG)-optimal Firefly luciferase - 3'UTR malat1 triple helixDepositorInsertSpaghetti monster (FLAG)-optimal FLuc - 3'UTR malat1 triple helix
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MCS-3AID-HA-2A-mTurquoiseBSD
Plasmid#225704PurposeEmpty backbone for lentiviral expression of AID degron tagged POI and mTurquoise fused BlasticidinRDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsT2A-mTurquoiseExpressionMammalianMutationPromoterpEF1AAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MCS-3AID-HA-2A-mCherryBSD
Plasmid#225700PurposeEmpty backbone for lentiviral expression of AID degron tagged POI and mCherry fused BlasticidinRDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsT2A-mCherryExpressionMammalianMutationPromoterpEF1AAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-rTetR(SE)-VP64-2A-mCherryBSD
Plasmid#225695PurposeLentiviral expression of rTetR(SE) fused to VP64 and mCherry fused BlasticidinRDepositorInsertrTetR-VP64
UseLentiviral and Synthetic BiologyTags3xFLAG and T2A-mCherryExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-rTetR(SE)-KRAB-2A-mTurquoise
Plasmid#225690PurposeLentiviral expression of rTetR(SE) fused to ZNF10 KRAB and mTurquoise-NLSDepositorInsertrTetR-ZNF10 KRAB
UseLentiviral and Synthetic BiologyTags3xFLAG and T2A-mTurquoiseExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only