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Plasmid#181793PurposepDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_humanIL-10_T2A_PDL1_PGK_mCherry
Plasmid#85430Purpose[Edit] Lentiviral vector for Gal4 inducible human IL-10/PD-L1 with constitutive mCherry expressionDepositorInserthuman IL-10 T2A PD-L1
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)
Plasmid#31476DepositorInsertSFFV d14 GFP donor EF1s I-SceI
UseLentiviralTagsHA and NLSExpressionMutationGFP has 14 amino acids deleted from c terminusPromoterAvailable sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFlag-CMV2-Smyd2
Plasmid#13521DepositorInsertSmyd2 (Smyd2 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET47b-T7-AsCas12f-WT
Plasmid#204640PurposeExpression of wild-type AsCas12f in E. coliDepositorInsertAsCas12f
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SMVP-Cas9N
Plasmid#80930PurposeExpresses Cas9N in mammalian cells.DepositorInsertCas9N
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb
Plasmid#175544PurposeExpression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cellsDepositorInsertIgKchL_HA-HaloTag9-myc_PDGFRtmb
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKM446 (Flag-DAS+4))
Plasmid#108321PurposeOrbit integrating plasmid for C-terminal tagging with degradation tag DAS+4, using hygromycin as a selection marker.DepositorInsertattB-Flag-DAS+4
UseOrbit integrating plasmidTagsExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETDuet S2
Plasmid#171921PurposeExpress RNA-based (p)ppGpp sensor and a NirFPDepositorInsertsRNA-based (p)ppGpp sensor
NirFP
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
10TALE_Pmin_fLuc
Plasmid#160663PurposeExpression of the firefly luciferase reporter upon binding of a transcriptional activator to target sites. The plasmid contains 10 copies of target sites for the TALEN1257 DNA-binding domainDepositorInsert10TALE_Pmin_fLuc
UseLuciferaseTagsExpressionMammalianMutationPromoterminimalAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPfetFn-Target_v2
Plasmid#199564PurposegRNA expression for FnCas12a in E. coli and clostridiaDepositorInsertSacB
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPthl_fUAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
flag-RapR-PTP1B
Plasmid#188656PurposeRapamycin regulated PTP1B with flag tagDepositorInsertPTP1B
UseTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMR-A-3
Plasmid#138517PurposeP3aDepositorInsertT7-RNAP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET14-Encap
Plasmid#86405PurposeExpresses T. maritima encapsulin in E. coliDepositorInsertEncapsulin
UseTagsExpressionBacterialMutationPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ef1a-DIO-eGCaMP(2+)
Plasmid#201150PurposeAAV virus production for Cre recombinase dependent expression of eGCaMP(2+)DepositorInserteGCaMP_2+
UseAAV, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDB302
Plasmid#113673Purposeexpresses Cth SUMO protease in Escherichia coliDepositorInsertCth SUMO protease
UseTags10-HisExpressionBacterialMutationdeleted amino acids 1-934PromoterT7Available sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
MK1274
Plasmid#71431PurposeThis E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.DepositorInsertMixed Feedback Loop version of the UBER system
UseTagsExpressionMutationT7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…PromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFH2.32_LV-mCherry-MS2x8-BCS
Plasmid#205539PurposeExpress mCherry-MS2x8 with barcodeDepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-Neq2X
Plasmid#182365PurposeExpresses Neq2X DNA polymeraseDepositorInsertNeq2X
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)
Plasmid#66708Purposeexpresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removedDepositorInsertS.Pyogenes VP64-dCas9-VP64
UseLentiviral and Synthetic BiologyTagsVP64ExpressionMutationD10A and H840APromoterhUbCAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only