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pJEP223-AAV-EFS-GFP-pA

Plasmid

#62913

Purpose

AAV vector backbone designed to express GFP from a EFS promoter. It also contains a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

AAV

Tags

Expression

Mutation

Promoter

short form of Elongation Factor1α (EFS)

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-Atoh1wt-IRES-GFP

Plasmid

#33333

Depositor

Charles Sherr, Martine Roussel

Article

Ayrault et al Cancer Res. 2010 Jul 1;70(13):5618-27. E

Insert

Atoh1 (Atoh1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
OCT4 GFP puro Donor 3

Plasmid

#22211

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Aug 13. ():.

Insert

SA-OCT-GFP-2APuro-PA (POU5F1 Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pRS306-pHIS3-2xGFP-tURA3

Plasmid

#116936

Purpose

Yeast integrative vector for the expression of 2xGFP

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

2xGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

HIS3

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac_HT_B_6xHis-TEV-GFP-NBR1

Plasmid

#190928

Purpose

Plasmid for the expression and purification of GFP labelled NBR1. Internal reference: SMC 914

Depositor

Sascha Martens

Article

Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

Insert

NBR1 (NBR1 Human)

Use

Tags

Strep-TEV-GFP

Expression

Insect

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CHAF1B_pLENTI-CAG-IRES-GFP

Plasmid

#177009

Purpose

Mammalian lentiviral expression vector encoding CHAF1B

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

CHAF1B (CHAF1B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSUM-Kan-MCS1-gfp

Plasmid

#109288

Purpose

Expresses high levels of gfp in mycobacteria

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

Multiple Cloning Site and GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Kanamycin

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pUAST mito roGFP2-Orp1

Plasmid

#64997

Purpose

for expression of mitochondrial roGFP2-Orp1 with the Gal4 expression system in drosophila

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Orp1 (HYR1 Budding Yeast)

Use

Tags

roGFP2

Expression

Insect

Mutation

mitochondrial targeting sequence

Promoter

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pAID1.2-EF1a-CGFP-mAID

Plasmid

#140608

Purpose

Expression of OsTIR1 and mAID-GFP protein in mammalian cells under the control of EF1a promoter

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

OsTIR1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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CAG-RORb2-IRES-GFPd2

Plasmid

#73998

Purpose

Plasmid expressing RORb2-IRES-GFPd2

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

RORb2 ORF (Rorb Mouse)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-SpyTag003 Loop A

Plasmid

#171776

Purpose

Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-SpyTag003 Loop A

Use

Tags

His6

Expression

Bacterial

Mutation

SpyTag003 and linkers inserted between residues V…

Promoter

T7

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe-cmv-hrGFP-zeo

Plasmid

#17714

Depositor

Susan Lindquist

Article

Dai et al Cell. 2007 Sep 21. 130(6):1005-18.

Insert

CMV-hrGFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pPRIME-TREX-GFP-FF3

Plasmid

#11667

Purpose

3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a Tet-responsive promoter (TREX) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

FF3

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pGFP-PTEN D92A/C124A

Plasmid

#50522

Depositor

Kenneth Yamada

Article

Tamura et al Science. 1998 Jun 5;280(5369):1614-7.

Insert

PTEN (PTEN Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

D92A/C124A

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pTNT-pT7-tetR-sfGFP

Plasmid

#140868

Purpose

T7 promoter driven synthesis of C-terminal fusion of sfGFP to TetR repressor (TetR-sfGFP)

Depositor

Neal Devaraj

Article

Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

Insert

TetR-sfGFP expressed with T7 promoter

Use

Synthetic Biology

Tags

sfGFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup93-mut

Plasmid

#87335

Purpose

To express human Nup93 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP93 (NUP93 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP C1-Eps8 ∆cap/∆bund

Plasmid

#74891

Purpose

mammalian expression of the capping and bundling activity mutant Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

Insert

Eps8 (Eps8 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

V689D, L693D, L757A, K759A

Promoter

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-Map7-Start-1351

Plasmid

#46075

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Map7-Start-1351 (Map7 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mCherry-ApoB-TagBFP

Plasmid

#129383

Purpose

Plasmid bearing the editing target of APOBEC1 fused between the mCherry and the Tag-BFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of Tag-BFP

Depositor

Silvestro Conticello

Article

Chieca et al Methods Mol Biol. 2021;2181:69-81. doi:

Insert

mCherry-ApoB-Tag-BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-mGFP-WDR34

Plasmid

#64251

Purpose

Expresses WDR34 as a fusion with monomeric GFP

Depositor

David Stephens

Article

Asante et al J Cell Sci. 2014 Nov 1;127(21):4774-87.

Insert

WDR34 (WDR34 Human)

Use

Lentiviral

Tags

mGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only