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Plasmid#104796PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g101120, Medtr2g101130 (Acre1). Also expresses Cas9 from Gmubi promoter from AtUBQ10 promoterDepositorInsertMedtr2g101120, Medtr2g101130
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC20
Plasmid#104794PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g020630 (FmoI). Also expresses Cas9 from AtUBQ10 promoterDepositorInsertMedtr2g020630
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC19
Plasmid#104793PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g020630 (FmoI). Also expresses Cas9 from Gmubi promoter from Gmubi promoterDepositorInsertMedtr2g020630
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC14
Plasmid#104788PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from AtUBQ10 promoterDepositorInsertMedtr2g044580
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC15
Plasmid#104789PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from rolD promoterDepositorInsertMedtr2g044580
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC13
Plasmid#104787PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr2g044580 (ERDJ2). Also expresses Cas9 from Gmubi promoterDepositorInsertMedtr2g044580
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRG312-slc1a-5
Plasmid#99102PurposedsRNA and riboprobe synthesis for Schmidtea mediterranea slc1a-5 (also known as excitatory amino acid transporter)DepositorInsertslc1a-5 (also known as excitatory amino acid transporter) in situ probe
UseT/a cloning vector for dsrna generation and the g…TagsExpressionMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC84-ScSEIPIN-Ndelta
Plasmid#96985PurposeExpress yeast SEIPIN gene in plants.DepositorInsertScSEIPIN (SEI1 Budding Yeast)
UseTags6xHis and GFPExpressionPlantMutationN-terminal 39 nucleotides were deleted from yeast…Promoter35SAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS315-PGK-SEIPIN2
Plasmid#96987PurposeExpress GFP-tagged Arabidopsis SEIPIN2 gene in yeast cells.DepositorInsertSEIPIN2 (AT1G29760 Mustard Weed)
UseTagsExpressionYeastMutationPromoterPhosphoglycerate Kinase (PGK) promoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC84-SEIPIN3N-1C
Plasmid#96983PurposeExpress mutated Arabidopsis SEIPIN1 gene in plants.DepositorInsertSEIPIN1 (AT5G16460 Mustard Weed)
UseTags6xHis and GFPExpressionPlantMutationA segment of 507 nucleotides from the N terminus…Promoter35SAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC84-SEIPIN1N-3C
Plasmid#96982PurposeExpress mutated Arabidopsis SEIPIN3 gene in plants.DepositorInsertSEIPIN3 (AT2G34380 Mustard Weed)
UseTags6xHis and GFPExpressionPlantMutationA segment of 108 nucleotides from the N terminus…Promoter35SAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC391
Plasmid#91220Purposeprotoplast vector expressing gRNA23 targeting tomato ANT1 (with TREX2 expression)DepositorInsertgRNA targeting tomato ANT1
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-D10A_1
Plasmid#91159PurposeCas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:hpt IIDepositorInsertAtCas9_D10A (nickase)
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC366
Plasmid#91215Purposeprotoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, ribozyme processing)DepositorInsert2 gRNAs targeting tomato ARF8A
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0808
Plasmid#91024PurposeModule A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoterEC1.2Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0408
Plasmid#91010PurposeModule A, Promoter: EC1.2, Gene: AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertAtCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoterEC1.2Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0708
Plasmid#91021PurposeModule A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_H840A nickase
UseCRISPRTagsExpressionMutationH840APromoterEC1.2Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSF_6His-ZmSUMO1a-GG_ZmSCE1d(C94S)-Myc
Plasmid#91935PurposeBacterial expression of maize SUMO1a and inactive SCE1dDepositorInserts6His-ZmSUMO1a-GG
ZmSCE1d(C94S)-Myc
UseTags6His and MycExpressionBacterialMutationC94S active site mutation and Truncation of ZmSUM…PromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSF_6His-ZmSUMO1a-GG_ZmSCE1b(C94S)-Myc
Plasmid#91933PurposeBacterial expression of maize SUMO1a and inactive SCE1bDepositorInserts6His-ZmSUMO1a-GG
ZmSCE1b(C94S)-Myc
UseTags6His and MycExpressionBacterialMutationC94S active site mutation and Truncation of ZmSUM…PromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
px330-MRPsg3
Plasmid#87191Purposedisruption of human RMRP gene via CRISPR-Cas9, guide 3DepositorInserthuman MRP RNA CRISPR guide #3 (RMRP Human)
UseCRISPRTagsExpressionMammalianMutationPromoterhuman U6Available sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only