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Plasmid#160252PurposeFor stable integration of FLAG-METTL3 in human cells with trasposaseDepositorInsertmethyltransferase like 3 (METTL3 Human)
UseTagsFLAGExpressionMammalianMutationPromotertight TRE promoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
KM-CashGem-Xyl2-URA3-LINEAR
Plasmid#174838PurposepRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianusDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastMutationPromoterTEF1pAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Sig10-Fc-IG
Plasmid#183722PurposeExpresses the fusion protein containing the extracellular part of human Siglec-10 and the Fc region of human IgG1, and GFPDepositorInsertSiglec-10
UseRetroviralTagsFc of human IgG1ExpressionMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-GLT1a-V5
Plasmid#162517PurposeRat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loopDepositorInsertGLT1a (Slc1a2 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-STAT2
Plasmid#178449Purposedoxycycline-inducible expression of Human STAT2 in mammalian cellsDepositorInsertSTAT2 (STAT2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-Y986S
Plasmid#174802PurposeBacterial expression of a PARP1 mutant that produces mainly short PAR oligomersDepositorInsertPARP1-Y986S (PARP1 Human)
UseTags6xHisExpressionBacterialMutationY986H, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-G972R
Plasmid#174800PurposeBacterial expression of a PARP1 mutant that produces less PAR oligomers overallDepositorInsertPARP1-G972R (PARP1 Human)
UseTags6xHisExpressionBacterialMutationG972R, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/mCherry-pBirA-HA
Plasmid#154458Purposeexpresses mCherry-pBirA-HA/biochemical assayDepositorInsertmCherry-pBirA-HA
UseTagsExpressionMammalianMutationPromoterCMV-F, T7 promoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV3Tag8li-hHP1BP3-HA
Plasmid#165468PurposeHuman HP1BP3 mammalian expression vector with C-terminal HA tag.DepositorInsertHeterochromatin protein 1 binding protein 3 (HP1BP3 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-SP110
Plasmid#178448Purposedoxycycline-inducible expression of Human SP110 in mammalian cellsDepositorInsertSP110 (SP110 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
bArr2-ALFA
Plasmid#201498PurposeExpression of the beta-arrestin2 containing a N-terminal ALFA tag.DepositorInsertbArr2-ALFA (ARRB2 Human)
UseTagsALFAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTV-Nanog_2-5prime-1000-24MS96T-3F_NeoR
Plasmid#174882PurposeTargeting vector for generating Nanog STREAMING-tag KI cellDepositorInsertSTREAMING-tag with homology arms for Nanog
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
YFP-FKBPx5-His
Plasmid#103785Purposeexpresses His-tagged iPOLYMER component in E. coli for protein purificationDepositorInsertYFP-FKBPx5-His
UseTagsEYFP, His-tagExpressionBacterialMutationPromoterT7Available sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9
Plasmid#84242PurposeExpresses GA-inducible KRAB-Sp dCas9DepositorInsertsGAI-tagBFP-Sp dCas9
GID1-KRAB
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…ExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
tdEos-VSVG-16
Plasmid#57693PurposeLocalization: Golgi/ER Transport, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertVSVG
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCALNL-Kir2.1
Plasmid#125578PurposeCre-dependent expression of Kir2.1 (FLAG-tagged) in mammalian cells.DepositorInsertKir2.1 (Kcnj2 Mouse)
UseCre/LoxTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-driven AcrIIC3-scaffold (2xBsmBI sites)
Plasmid#120301PurposeAAV vector for expression of AcrIIC3 (no miR binding sites, control vector)DepositorInsertAcrIIC3
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Poly(A)
Plasmid#65524PurposePlasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-GFP
UseMouse Targeting; Bxb1TagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Zeo-TTF-1
Plasmid#75089Purposeretrovirus-mediated gene transfer into mammalian cellsDepositorInsertTTF-1 (NKX2-1 Human)
UseRetroviralTagsnoneExpressionMammalianMutationwild-type, full-length, without native 3' UTRPromoterAvailable sinceMay 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
TIA1 RRM-YFP-SspBx6
Plasmid#103781Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertTIA1 RRM-YFP-SspBx6
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only