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pDONR223_NRAS_WT

Plasmid

#82150

Purpose

Gateway Donor vector containing NRAS , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NRAS (NRAS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTPN12_p.E5G

Plasmid

#81532

Purpose

Gateway Donor vector containing PTPN12 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTPN12 (PTPN12 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E5G; V322I

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGS-FCER1G-1

Plasmid

#109192

Purpose

Encodes human full-length FCER1G to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

Human full-length FCER1G, codon-optimized (FCER1G Human)

Use

Tags

Expression

Insect

Mutation

Promoter

p10

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_SAMHD1_p.I515T

Plasmid

#81506

Purpose

Gateway Donor vector containing SAMHD1 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SAMHD1 (SAMHD1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G404E; I515T; A546V

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_VHL_p.S111N

Plasmid

#81675

Purpose

Gateway Donor vector containing VHL, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

VHL (VHL Human)

Use

Gateway entry vector

Tags

Expression

Mutation

S111N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CYP3A5_WT

Plasmid

#81823

Purpose

Gateway Donor vector containing CYP3A5 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CYP3A5 (CYP3A5 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_NFKBIA_p.P137S

Plasmid

#81367

Purpose

Gateway Donor vector containing NFKBIA , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NFKBIA (NFKBIA Human)

Use

Gateway entry vector

Tags

Expression

Mutation

S76P; P137S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP C1-Eps8 ∆cap/∆bund

Plasmid

#74891

Purpose

mammalian expression of the capping and bundling activity mutant Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

Insert

Eps8 (Eps8 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

V689D, L693D, L757A, K759A

Promoter

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
pDONR223_PRKAA1_WT

Plasmid

#82274

Purpose

Gateway Donor vector containing PRKAA1, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PRKAA1 (PRKAA1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

M10L

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM10_WT

Plasmid

#81958

Purpose

Gateway Donor vector containing RBM10, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RBM10 (RBM10 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pdCas9-M-C4

Plasmid

#73427

Purpose

CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant C4.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

Repressor C4 (orthogonal T7-lac repressor)

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Constitutive wild-type S. pyogenes promoter

Available since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_p.E47A

Plasmid

#81645

Purpose

Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E47A

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-Cherry-AnillinA740D, E758K

Plasmid

#187273

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758K

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Anillin A740D, E758K

Promoter

U6, CMV

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_STK19_WT

Plasmid

#81919

Purpose

Gateway Donor vector containing STK19 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STK19 (STK19 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_VHL_p.H115Y

Plasmid

#81658

Purpose

Gateway Donor vector containing VHL, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

VHL (VHL Human)

Use

Gateway entry vector

Tags

Expression

Mutation

H115Y

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAT6_p.D46N

Plasmid

#81537

Purpose

Gateway Donor vector containing STAT6 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT6 (STAT6 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

D46N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_VHL_p.R161P

Plasmid

#81671

Purpose

Gateway Donor vector containing VHL, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

VHL (VHL Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R161P

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3R1_WT

Plasmid

#82310

Purpose

Gateway Donor vector containing PIK3R1 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3R1 (PIK3R1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E181K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NLS-YFP-CCTd

Plasmid

#184325

Purpose

Mammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.3)

Depositor

Xiaodong Liu

Article

Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

Insert

CCTd (CACNA1D Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTPN12_p.E57D

Plasmid

#81528

Purpose

Gateway Donor vector containing PTPN12 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTPN12 (PTPN12 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E57D; V322I

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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