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Plasmid#230996PurposeProtein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.DepositorTypeEmpty backboneUseTagsAcGFP1ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only
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pMS19
Plasmid#229475PurposeExpresses RssB with E135A, E136A, & E137A mutationDepositorInsertRssB (E135A, E136A, E137A)
UseTagsExpressionBacterialMutationE135A, E136A, E137APromoterT7Available sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMS21
Plasmid#229476PurposeExpresses RssB with E135A, E136A, & E137A mutation with His-tagDepositorInsertRssB (E135A, E136A, E137A)
UseTags10xHisExpressionBacterialMutationE135A, E136A, E137APromoterT7Available sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVF18
Plasmid#229456PurposeExpresses IraD (aa17-130) with His-tagDepositorInsertIraD (aa 17-130 only)
UseTags6xHisExpressionBacterialMutationaa 17-130 onlyPromoterT7Available sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTYC1_pPromALB_enh5898_FireflyLuc
Plasmid#220285PurposeFirefly luciferase with mouse liver DNase hypersensitive site # 5898 cloned into KpnI/XhoI-digested pPromALB_FireflyLucDepositorInsertmouse liver DNase hypersensitive site # 5898
UseLuciferaseTagsExpressionMutationPromoterminimal albumin promoterAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTYC2_pPromALB_enh29774_FireflyLuc
Plasmid#220286PurposeFirefly luciferase with mouse liver DNase hypersensitive site # 29774 cloned into KpnI/XhoI-digested pPromALB_FireflyLucDepositorInsertmouse liver DNase hypersensitive site # 29774
UseLuciferaseTagsExpressionMutationPromoterminimal albumin promoterAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTYC3_pPromALB_enh50363_FireflyLuc
Plasmid#220287PurposeFirefly luciferase with mouse liver DNase hypersensitive site # 50363 cloned into KpnI/XhoI-digested pPromALB_FireflyLucDepositorInsertmouse liver DNase hypersensitive site # 50363
UseLuciferaseTagsExpressionMutationPromoterminimal albumin promoterAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTYC4_pPromALB_enh50358_FireflyLuc
Plasmid#220288PurposeFirefly luciferase with mouse liver DNase hypersensitive site # 50358 cloned into KpnI/XhoI-digested pPromALB_FireflyLucDepositorInsertmouse liver DNase hypersensitive site # 50358
UseLuciferaseTagsExpressionMutationPromoterminimal albumin promoterAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGYL17_pPromALB_enhALB_FireflyLuc
Plasmid#220283PurposeFirefly luciferase with minimal Albumin promoter with a 380 nt, -10.5 kb mouse Alb enhancer cloned into KpnI/XhoI-digested pPromALB_FireflyLucDepositorInsertAlb Enhancer
UseLuciferaseTagsExpressionMutationPromoterminimal albumin promoterAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGYL18_pPromALB_enhALB_RenillaLuc
Plasmid#220284PurposeRenilla luciferase with minimal Albumin promoter with a 380 nt, -10.5 kb mouse Alb enhancer cloned into KpnI/XhoI-digested pGL4.70 (Promega)DepositorInsertAlb Enhancer
UseLuciferaseTagsExpressionMutationPromoterminimal albumin promoterAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro
Plasmid#195567PurposePiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cellsDepositorInsertTIR1
UseTagsExpressionMammalianMutationPromoterEf1-alphaAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hfYFP-TEV-Bcx
Plasmid#191195PurposeFusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInserthfYFP & Bcx fusion
UseTags6xHis and Bacillus circulans xylanaseExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-T7-VHH-GFPminimizer
Plasmid#202488PurposeExpresses the nanobody GFP minimizer with T7 inductionDepositorInsertnanobody GFP minimizer
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH72
Plasmid#202467PurposeExpresses the nanobody VHH72 after phosphate depletionDepositorInsertnanobody VHH72
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-H6antivenin
Plasmid#202470PurposeExpresses the nanobody H6 antivenin after phosphate depletionDepositorInsertnanobody H6 antivenin
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-VCAM1
Plasmid#202471PurposeExpresses the nanobody VCAM1 after phosphate depletionDepositorInsertnanobody VCAM1
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-1B5
Plasmid#202472PurposeExpresses the nanobody 1B5 after phosphate depletionDepositorInsertnanobody 1B5
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-2E7
Plasmid#202473PurposeExpresses the nanobody 2E7 after phosphate depletionDepositorInsertnanobody 2E7
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-3E3
Plasmid#202474PurposeExpresses the nanobody 3E3 after phosphate depletionDepositorInsertnanobody 3E3
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-yibDp-VHH-C9BoNT-A
Plasmid#202475PurposeExpresses the nanobody C9 BoNT-A after phosphate depletionDepositorInsertnanobody C9 BoNT/A
UseTags6X-HisExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only