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LV_EF1a_KRAS(G12V)-P2A-Hygro_Barcode

Plasmid

#170237

Purpose

Barcoded lentiviral vector to express KRAS (G12V) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

KRAS (KRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

G12V

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pmiRFP-N1

Plasmid

#108409

Purpose

Monomeric near-infrared fluorescent protein miRFP in mammalian expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

miRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-EF1a-Archon1-KGC-EGFP-ER2

Plasmid

#108425

Purpose

Lentivirus production plasmid encoding for Archon1 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-EGFP-ER2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-sgHcn1#2/Cre

Plasmid

#193218

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Hcn1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgHcn1#2 (Hcn1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Archon2-KGC-GFP-ER2-WPRE

Plasmid

#108424

Purpose

Archon2 fluorescent voltage reporter in mammalian expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon2-KGC-EGFP-ER2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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mRFP-FKBP12-5ptpase domain

Plasmid

#67516

Purpose

Recruitable human type IV 5-phosphatase domain (214-644)

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)

Use

Tags

mRFP

Expression

Mutation

INPP5E has C641A to destroy the C-terminal CAAX d…

Promoter

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
Anti-HCN1 [N70/28R]

Plasmid

#114512

Purpose

Mammalian Expression Plasmid of anti-HCN1 (Rat). Derived from hybridoma N70/28.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-HCN1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Hcn1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-hATG7CS

Plasmid

#87868

Purpose

Express a active-site mutant human Atg7/Apg7 C572S in mammalian cells

Depositor

Isei Tanida, Eiki Kominami

Article

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

ATG7 C572S mutant (ATG7 Human)

Use

Tags

Expression

Mammalian

Mutation

C572S mutant

Promoter

CMV

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-Nr4a1 double gRNAs

Plasmid

#162791

Purpose

Multiplex Guide RNAs to generate Nr4a1 knockout by CRISPR

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Multiplex Nr4a1 gRNA 1 and 2 (Nr4a1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-EF1a-Archon2-KGC-EGFP-ER2

Plasmid

#108426

Purpose

Lentivirus production plasmid encoding for Archon2 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon2-KGC-EGFP-ER2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Archon1-EGFP

Plasmid

#108417

Purpose

AAV production plasmid encoding for Archon1 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CaMKII

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pSF143-wArchon1-KGC-EGFP-ER2

Plasmid

#108429

Purpose

Codon-optimized Archon1 fluorescent voltage reporter in C.elegans expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

wArchon1-KGC-EGFP-ER2

Use

Tags

Expression

Worm

Mutation

Promoter

flp-18

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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mRFP-FKBP12-5ptpase enzyme

Plasmid

#67515

Purpose

Recruitable human type IV 5-phosphatase enzyme

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

INPP5E has C641A to destroy the C-terminal CAAX d…

Promoter

CMV

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)

Plasmid

#169336

Purpose

Expression of the LT-Fucci(SA) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(SA) (GMNN, CDT1 Human)

Use

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk

Plasmid

#131143

Purpose

Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

mNeonGreen::3XFLAG::Crk (Crk Fly)

Use

Cre/Lox; Attb recombination site

Tags

3XFLAG and mNeonGreen

Expression

Insect

Mutation

Promoter

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-SAP97 [K64/15R]

Plasmid

#128624

Purpose

Mammalian Expression Plasmid of anti-SAP97 (Rat). Derived from hybridoma K64/15.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SAP97 (Rat) recombinant mouse monoclonal antibody (Dlg1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-MMP9 matrix metalloproteinase-9 precursor [L51/82R]

Plasmid

#149455

Purpose

Mammalian Expression Plasmid of anti-MMP9 matrix metalloproteinase-9 precursor (Rat). Derived from hybridoma L51/82.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-MMP9 matrix metalloproteinase-9 precursor (Rattus norvegicus) recombinant mouse monoclonal antibody (Mmp9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pmiRFP-beta-Actin-C-19

Plasmid

#108412

Purpose

Beta-actin fused to monomeric near-infrared fluorescent protein miRFP

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

beta-Actin

Use

Tags

miRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/Halo-Tag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-100)Cy+

Plasmid

#169337

Purpose

Expression of the LT-Fucci(SCA) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(SCA) (GMNN, CDT1 Human)

Use

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-100)Cy…

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG1_CH3-3

Plasmid

#105853

Purpose

Expression plasmid coding for hybrid heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG3-derived CH3.

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG1 heavy chain, IgG3 heavy chain (Ighg1, Ighg3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

hybrid heavy chain of mouse IgG1 with CH3 derived…

Promoter

hEF1-HTLV prom

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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