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Plasmid#109033PurposeD16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
UseTagsFlag TagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJZC35
Plasmid#62325PurposesgRNA for mammalian cells with mCherry markerDepositorInsertsgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_HLF
Plasmid#86262PurposeDonor vector for 3' FLAG tag of human HLFDepositorInsertHLF homology arms (HLF Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJZC102
Plasmid#62337PurposesgRNA for mammalian cells with mCherry markerDepositorInsertsgRNA
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
Plasmid#222990PurposeBacterial expression of iGeoCas9(C1) constructDepositorInsertHis-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
UseCRISPRTagsCL7, His, and MBPExpressionBacterialMutationPromotertacAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
p944-SWITCH-OVER insert: EF1a-Puro
Plasmid#217891Purposeinsert vector for Switch-OVER: Single loxP-STOP-EF1a-Puro-mU6DepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
p931-SWITCH-OVER insert: EF1a-Blasti
Plasmid#217890Purposeinsert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6DepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262F-ABE8e
Plasmid#199179PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e-HF mediated A-G base editingDepositorInsertABE8e(V106W)-zSpCas9(D10A)
UseCRISPR; Gateway compatible abe8e(v106w)-zspcas9(d…TagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgCIDE-10_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211670PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgCIDE-10 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgCIDE-5_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211665PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgCIDE-5 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgCIDE-6_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211666PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgCIDE-6 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgCIDE-7_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211667PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgCIDE-7 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgRPA1-2_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211659PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgRPA1-2 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgCIDE-4_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211664PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgCIDE-4 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgCIDE-21_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211671PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgCIDE-21 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgCIDE-22_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211672PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgCIDE-22 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgOR2B6-1_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211655PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgOR2B6-1 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgOR2B6-2_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211656PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgOR2B6-2 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgRPA1-3_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211660PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgRPA1-3 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF821-sgCIDE-2_U6-sgRNA-EF1a-mNeonGreen
Plasmid#211662PurposeU6-sgRNA-EF1a-mNeonGreenDepositorInsertSpyCas9 and sgCIDE-2 guide RNA vector
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only