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Plasmid#196352PurposeExpression of mAzurite mRNA with 8xbiRhoBAST-24xMS2v6 in 3'UTRDepositorInsertmAzurite-8xbiRhoBAST-24xMS2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDNR-mCherry-T2A-3xPuroR-Frame 1
Plasmid#206992PurposeDonor plasmid for Frame 1 with mCherry tag and triple Puromycin resistant marker (3xPuroR)DepositorTypeEmpty backboneUseSynthetic BiologyTagsmCherryExpressionMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDNR-mCherry-T2A-3xPuroR-Frame 2
Plasmid#206993PurposeDonor plasmid for Frame 2 with mCherry tag and triple Puromycin resistant marker (3xPuroR)DepositorTypeEmpty backboneUseSynthetic BiologyTagsmCherryExpressionMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
NM9-SpCas9-NLS3
Plasmid#128177PurposeExpresses R. toruloides codon-optimized SpCas9 under PGK1 promoterDepositorInsertSpCas9-NLS3
UseCRISPRTagsExpressionYeastMutationPromoterpPGK1Available sinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330S-3
Plasmid#58779PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_PGK_mCherry
Plasmid#85434PurposeLentiviral vector for Gal4 inducible leucinezipper Pembrolizumab and constitutive mCherry expressionDepositorInsertPembrolizumab
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_humanIL-10_IRES_mCherry_PGK_tBFP
Plasmid#85427PurposeLentiviral vector for Gal4 inducible humanIL-10 with mCherry reporter and constitutive tBFP expressionDepositorInserthuman IL-10
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGNW2
Plasmid#122086Purposesuicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cellsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEBFP2-Nuc
Plasmid#14893PurposeMammalian expression of EBFP2 blue fluorescent protein with NLSDepositorInsertpEBFP2-Nuc
UseTagsNLSExpressionMammalianMutationReplace EYFP of the vector pEYFP-Nuc from Clontec…PromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
bactin2:loxP-STOP-loxP-DsRed-express
Plasmid#68797PurposeFor making transgenic fishDepositorInsertsbeta-actin promoter
loxp-stop-codon-loxp-dsred
UseTagsExpressionMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-8xbiRhoBAST
Plasmid#196351PurposeExpression of mEGFP mRNA with 8xbiRhoBAST in 3'UTRDepositorInsertmEGFP-8xbiRhoBAST
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
MK1283
Plasmid#71428PurposeThis E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709DepositorInsertMixed Feedback Loop version of the UBER system
UseTagsExpressionMutationT7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …PromoterAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_Blinatumomab_PGK_mCherry
Plasmid#85437Purpose[Edit] Lentiviral vector for Gal4 inducible Blinatumomab and constitutive mCherry expressionDepositorInsertBlinatumomab
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC1.509
Plasmid#203955PurposeLevel 1 self-replicating vector containing a DAPG inducible FnCas12aDepositorInsertPphlF-Cpf1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
gCH128 (non-targeting crRNA)
Plasmid#217339PurposeNon-targeting crRNADepositorInsertcrNT-3
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ142-ZmUbi-tRNA
Plasmid#158578PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 and pYPQ132-tRNA2.0; assembly of 2 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE
Plasmid#101104PurposeEncodes dCas9(N) fused to VEGFR2DepositorInsertVEGFR2-dCas9(N)
UseCRISPR and Synthetic BiologyTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-NanoLuc
Plasmid#188313PurposeExpression of NanoLuc via a T7 promoter in TXTLDepositorInsertNanoLuc
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialMutationPromoterT7MaxAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-polysite
Plasmid#122843PurposeGateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.DepositorTypeEmpty backboneUseGateway destinationTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pS448∙CsR
Plasmid#122096PurposeCRISPR/Cas9 counterselection in Gram-negative bacteriaDepositorInsertsCas9
sgRNA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPem7 and PmAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only