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pHBS1415 GFP-TDP43 VLIM-S

Plasmid

#133323

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mutation

ALL VLIM in the TDP43 IDRs mutated to S

Promoter

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO GFP MPS1 K-R

Plasmid

#59819

Purpose

Allows the integration of GFP MPS1 K-R in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Tighe et al J Cell Biol. 2008 Jun 16;181(6):893-901.

Insert

Mps1 (TTK Human)

Use

Tags

EmGFP

Expression

Mammalian

Mutation

KD, catalytically inactive, D664A

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pXGFPC-5 Pxyl-dcas9 (Spy)

Plasmid

#133316

Purpose

expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

dcas9 (Streptococcus pyogenes)

Use

CRISPR

Tags

Expression

Mutation

Promoter

xylose

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCLXSN(GFP)-myc-BioID-C

Plasmid

#174737

Purpose

Overexpresion

Depositor

Shao-Cong Sun

Article

Li et al Cell Res. 2019 Jun;29(6):474-485. doi: 1

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-GFPIvy-SpHis5

Plasmid

#60783

Purpose

Yeast C-terminal tagging vector, which creates gene fusions with GFPIvy using SpHis5 selection

Depositor

Linda Huang

Article

Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

Insert

GFPIvy

Use

Yeast integrating

Tags

Expression

Mutation

S30R Y39N T65C Q69A S72A F99S N105T Y145F M153T V…

Promoter

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Tet1-Poly(A)

Plasmid

#65559

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Tet1 cDNA into a MIN-tagged locus

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Tet1 (Tet1 Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-N-gamma-tubulin (1-333)

Plasmid

#87858

Purpose

Fluorescent fragment of gamma-tubulin that lacks gamma-tubulin DNA binding domain and NLS

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

gamma-tubulin 1 (TUBG1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Fragment from aa 1 to 333

Promoter

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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KP201: pMVP (L5-L4) eGFP-P2A

Plasmid

#121708

Purpose

pMVP L5-L4 entry plasmid, contains eGFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eGFP linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP-P2A (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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BAS286 C terminal SF-GFP hygBR

Plasmid

#74081

Purpose

S.pombe recoded superfolder GFP in universal C-terminal tagging plasmid, hygB resistanc

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

super-folder GFP

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
M71-IRES-tauGFP LTNL TV

Plasmid

#15639

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

M71-IRES-tauGFP LTNL TV

Use

High copy number

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pEGFP-C3-PLK4-S305E-3xFLAG

Plasmid

#69840

Purpose

This plasmid encodes PLK4 isoform 1 carrying an S305E mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Use

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

S305E

Promoter

CMV

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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p{ie1-eGFP::FRT-RC,attB}

Plasmid

#182175

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLP-hSox2-ires-gfp

Plasmid

#49391

Purpose

mammalian bicistronic expression of human Sox2 and EGFP

Depositor

Randall McKinnon

Insert

Sox2 (SOX2 Human)

Use

Cre/Lox; Acceptor vector

Tags

6xHN, EGFP, and IRES

Expression

Mammalian

Mutation

TGA (stop) to CCA (Pro) at nt 1027 with respect t…

Promoter

CMV

Available since

Dec. 2, 2013

Availability

Academic Institutions and Nonprofits only
pLP-mOlig2-ires-gfp

Plasmid

#49399

Purpose

mammalian bicistronic expression of mouse Olig2 and EGFP

Depositor

Randall McKinnon

Insert

Olig2 (Olig2 Mouse)

Use

Cre/Lox; Acceptor vector

Tags

EGFP and IRES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-132-Decoy.hPGK.GFP.WPRE

Plasmid

#46587

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-132 Decoy (MIR132, Mir132 Mouse, Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
JT504: pMVP/SB/eGFP-DEST

Plasmid

#121861

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eGFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-ULK2(K39T)

Plasmid

#38206

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

Unc-51 like kinase 2 (Ulk2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed Lysine 39 to Threonine

Promoter

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
ATF4 18: RLuc.5’ATF4.GFP

Plasmid

#21864

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only
cmv-sfGFP-GB1-scAB

Plasmid

#185794

Purpose

scFv GFP compnent for SunRISER

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

sfGFP-GB1-scAB

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;clecl14aCE1-P1Egfp

Plasmid

#90157

Purpose

contains clec14a enhancer sequence, and clec14a specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

clec14aE1P1Egfp

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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