Addgene

We narrowed to 24,236 results for: sta

Showing: 8461 - 8480 of 24236 results

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pSUPER-retro-puro-shNup88-HindIII

Plasmid

#87329

Purpose

To express shRNA against human Nup88

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup88

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-bSV-831-1792

Plasmid

#40961

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-830

Promoter

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-bSV-1010-1792

Plasmid

#40959

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-1009

Promoter

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
pMOD_A1310

Plasmid

#91034

Purpose

Module A, Promoter: ZmUbi, Gene: TaCas9_H840A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_H840A nickase

Use

CRISPR

Tags

Expression

Mutation

H840A

Promoter

ZmUbi

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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mEos2-CENPB-N-22

Plasmid

#57360

Purpose

Localization: Nucleus/Centromeres, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CENPB (CENPB Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMOD_A0208

Plasmid

#91004

Purpose

Module A, Promoter: EC1.2, Gene: AtCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A nickase

Use

CRISPR

Tags

Expression

Mutation

D10A

Promoter

EC1.2

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEG587

Plasmid

#40196

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (S458A) (RNAi resistant) (mex-5 Nematode)

Use

Tags

Dendra2 PAFP (sequence optimized for worm express…

Expression

Worm

Mutation

S458A; exon 2 recoded to be RNAi-resistant

Promoter

4.4 kb mex-5 promoter fragment

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pENTR221-WNT3A

Plasmid

#79501

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3A CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WNT3A (WNT3A Human)

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

no Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Flag3-pG-cNanog-pgk-hph

Plasmid

#74912

Purpose

Mammalian expression of Flag tagged cNanog

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAB043a

Plasmid

#79222

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ lambda cI-MBP; PlacIq lacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ; PlacIq

Available since

April 15, 2017

Availability

Academic Institutions and Nonprofits only

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pAG416GPD-Cerulean-VPS21

Plasmid

#18842

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

VPS21 (VPS21 Budding Yeast)

Use

Tags

Cerulean

Expression

Yeast

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pAB082f

Plasmid

#79238

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-Cry1Ac-d23A

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ppro1

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pSG 415

Plasmid

#63862

Purpose

Detect translation initiation from AUC start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

AUC start codon

Promoter

PtacI

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only
pSG 414

Plasmid

#63861

Purpose

Detect translation initiation from UUG start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

UUG start codon

Promoter

PtacI

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pSG 431

Plasmid

#63866

Purpose

Detect translation initiation from GUG start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

GUG start codon

Promoter

PtacI

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pJK511

Plasmid

#71591

Purpose

Produces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag, His222>Gln mutant (TpCSH6-H222Q)

Depositor

T. J. Kappock

Article

Murphy et al Acta Crystallogr F Struct Biol Commun. 2

Insert

citrate synthase

Use

Tags

His6

Expression

Bacterial

Mutation

changes histidine-222 to glutamine

Promoter

T7 promoter

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-S387A

Plasmid

#33071

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S387A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
FusionRed-CAF1-C-10

Plasmid

#56102

Purpose

Localization: Nucleus, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

CAF1

Use

Tags

FusionRed

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-EB1-C-18

Plasmid

#57371

Purpose

Localization: MT End Binding Protein, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

EB1 (MAPRE1 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
RCAS (A) Caronte (CT#382)

Plasmid

#13862

Depositor

Cliff Tabin

Article

Yokouchi et al Cell. 1999 Sep 3. 98(5):573-83.

Insert

Caronte (CER1 Chicken)

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only