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Plasmid#195861PurposeEncodes chromogenic protein (CP) with nonsense mutation resulting in amber stop codon at amino acid 62. CP inducible with rhamnose in amber suppressor strains.DepositorInsertAmber Chromogenic Protein on pRCPam plasmid
UseTagsExpressionBacterialMutationGln62XPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SNAP-CRY2-VHH(GFP)
Plasmid#58370PurposeFor use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MPDepositorInsertCRY2[1-498] (CRY2 Mustard Weed)
UseTagsSNAP and vhhGFP4ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQFlag-USP11 CS puroR
Plasmid#46748PurposeRetroviral vector that expresses catalytically inactive form of Flag-tagged human USP11DepositorInsertUSP11 (USP11 Human)
UseRetroviralTagsFlagExpressionMammalianMutationC318S--catalytically inactivePromoterCMVAvailable sinceMarch 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6.gDNMT1.Cas9-T2A-GFP
Plasmid#170362PurposePlasmid used for Crispr/cas9 based disruption of human DNMT1.DepositorInsertCas9-T2A-GFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pyc111m
Plasmid#158779Purposeribosome-tagged, Cre-dependent GCaMP for soma localizationDepositorInsertGCaMP6m-RPL10a
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-EGFP-C1-Flag-mFmr1(A)
Plasmid#87913PurposeExpresses mouse FMRP-EGFP fusion with S499A to prevent phosphorylation.DepositorInsertFmr1 (Fmr1 Mouse)
UseTagsFlag and eGFPExpressionMammalianMutationS499APromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUF1-dCas9-GCN5
Plasmid#122509PurposeCombining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.DepositorInsertdCas9-GCN5
UseCRISPRTags3*FLAGExpressionMutationdCas9(D10A,H840A)PromoterPfHsp86Available sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pdCASclos
Plasmid#73640PurposeTranscriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824DepositorInsertsdCas9
sgRNA to spo0A
UseE.coli-clostridium shuttle vectorTagsExpressionMutationD10A, H840APromoterPj23119 and ptbAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
MO70-mJP-4
Plasmid#79598PurposeTransient and retroviral expressionDepositorInsertJunctophilin-4 (Jph4 Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMy-CTCF-GFP
Plasmid#50574PurposeRetroviral vector endoding for mouse CTCF fused to GFPDepositorInsertAmpicilin
UseRetroviralTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Tol2(Chien)-Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA
Plasmid#105643PurposeCo-expression of tdTomato and HyPer in differentiated keratinocytes of the periderm layer and ealy on also in the basal layer of the epidermisDepositorInsertKrt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pHS1929
Plasmid#205968PurposeJAGBLH010000220 Cas8-HNH crRNA expression in pCOLADuet-1DepositorInsertCas8-HNH crRNA
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
AsCas12a-DR-cr in PBCSK-
Plasmid#126640PurposeCloning vector AsCas12a DR-crRNA for expression in mammalian cellsDepositorInsertAsCas12a Full Length Direct Repeat crRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A hu_ncOGT WT
Plasmid#154281PurposeEntry vector encoding non-tagged O-GlcNAc TransferaseDepositorInsertO-GlcNAc Transferase (OGT Human)
UseGateway entry vectorTags2x FLAG and mKate2ExpressionMutationWT OGTPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 c-SRC (151-536)
Plasmid#42207DepositorInsertc-SRC (151-536) (SRC Human)
UseTagsExpressionMammalianMutationcontains amino acids 151-536PromoterCMVAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NUP188
Plasmid#159893PurposeExpresses NUP188 in mammalian cellsDepositorInsertNUP188 (NUP188 Human)
UseTagsEGFPExpressionMammalianMutationTo avoid NotI site, first three amino acids are d…PromoterCMVAvailable sinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
ins:MCS; cryaa:RFP
Plasmid#110282PurposeA zebrafish insulin promoter containing vector with multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites.DepositorTypeEmpty backboneUseZebrafishTagsExpressionMutationPromoterinsulinAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHS1068
Plasmid#205962PurposeNZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promotersDepositorInsertDinG HNH proteins and CRISPR array
UseTagsExpressionBacterialMutationPromoterLac (proteins), pJ23119 (crRNA)Available sinceFeb. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 c-SRC (1-397)
Plasmid#42205DepositorInsertc-SRC (1-397) (SRC Human)
UseTagsHAExpressionMammalianMutationcontains amino acids 1-397PromoterCMVAvailable sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only