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Plasmid#135617PurposeDonor vector for genomic targeting of a P2A-mStrawberry cassette to the 3' end of the mouse Sox2 locusDepositorInsertP2A-mStrawberry flanked by Sox2 homology arms (Sox2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsP2A-mStrawberryExpressionMutationPromoterAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBMN mEGFP-NDP52
Plasmid#188785PurposeFor mammalian expression of NDP52 tagged with mEGFPDepositorInsertNDP52 (CALCOCO2 Human)
UseRetroviralTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-CoRESTfl
Plasmid#109156PurposeEncodes human full-length CoREST with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length CoREST, sequence-optimized for insect cells (RCOR1 Human)
UseTags3xFLAGExpressionInsectMutationPromoterPolyhedrinAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
amilCP_Pink chromoprotein
Plasmid#117851PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Pink chromoprotein in E. coliDepositorInsertpromoter, RBS, amilCP_Pink
UseSynthetic Biology; Escherichia coliTagsExpressionMutation64A,65C, BioBrick sites removedPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-eGFP-P2A-LgBiT-NLS
Plasmid#187834PurposeExpression of eGFP-P2A-LgBiT-NLS from human synapsin promoter via adeno-associated virus for neuronal expression.DepositorInserteGFP-P2A-LgBiT-NLS
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEL032
Plasmid#137901PurposeSTU2.0 LbCas12a for plant genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHLP700
Plasmid#203742PurposeC-terminal tagging of target proteins with AID*/3xHA degronDepositorInsertIAA17 auxin binding domain codons 71-114
UsePcr amplification template for gene taggingTags3xHAExpressionMutationPromoternoneAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-LIN-41
Plasmid#52716Purposeretroviral expression of human LIN-41 / TRIM71DepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mGold-Calnexin-N-14
Plasmid#158004PurposeVisualization of ER in mammalian cells using mGoldDepositorInsertCalnexin (CANX Human)
UseTagsmGoldExpressionMammalianMutationI474NPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
beta Synuclein-VC
Plasmid#89484Purposeexpresses human beta Synuclein fused to the C-terminal part of VenusDepositorInsertSNCB (SNCB Human)
UseTagsc-terminal part of VenusExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-GST-LC3B
Plasmid#216836PurposeFor expression in E. coli of GST-LC3B (Gly exposed) (Internal ref. SMC1463)DepositorInsertLC3B (Gly exposed) (MAP1LC3B Human)
UseTagsGSTExpressionBacterialMutationPromoterTAC promoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS4S
Plasmid#55170PurposeEmpty vector, integration at thrC, ampr, specrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoternoneAvailable sinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
POLArISact
Plasmid#164971PurposeExpresses POLArISact, an orientation probe that specifically binds to F-actin, in mammalian cellsDepositorInsertPOLArISact
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pv6_MBD1_MBD
Plasmid#179404PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBDDepositorInsertMBD1_MBD
UseTagsAviTag and EGFPExpressionMammalianMutationPromoterCAGGSAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pyc111f
Plasmid#158780Purposeribosome-tagged, Cre-dependent GCaMP for soma localizationDepositorInsertGCaMP6f-RPL10a
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lyn FAK biosensor
Plasmid#78299PurposeFRET biosensor. Visualization of FAK activity at lipid rafts in live cellsDepositorInsertLyn Fak biosensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
TIA1 RRM-mCherry-iLIDx6
Plasmid#103782Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertTIA1 RRM-mCherry-iLIDx6
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIDS-RESTwt full-length
Plasmid#109161PurposeEncodes human full-length REST/NRSF to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length REST/NRSF (REST Human)
UseTagsExpressionInsectMutationPromoterp10Available sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHS1543
Plasmid#205963PurposeNZ_CP025815 DinG HNH Cascade proteins operon (E. coli codon optimized) with His14-tagged Csf3 in pET45b(+)DepositorInsertDinG HNH Cascade with His14-tagged Csf3
UseTags14xHis on Csf3ExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
ALFA-bArr1
Plasmid#201495PurposeExpression of the beta-arrestin1 containing a N-terminal ALFA tag.DepositorInsertALFA-bArr1 (ARRB1 Human)
UseTagsALFAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only