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pDONR223_U2AF1_WT

Plasmid

#81954

Purpose

Gateway Donor vector containing U2AF1, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

U2AF1 (U2AF1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_WT

Plasmid

#81737

Purpose

Gateway Donor vector containing PTEN , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_REG1A_WT

Plasmid

#81785

Purpose

Gateway Donor vector containing REG1A , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

REG1A (REG1A Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TNFRSF14_WT

Plasmid

#81850

Purpose

Gateway Donor vector containing TNFRSF14 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TNFRSF14 (TNFRSF14 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

K17R

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP

Plasmid

#173029

Purpose

For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.

Depositor

Mohamed Fareh

Article

Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

Insert

pspCas13b

Use

CRISPR and Lentiviral

Tags

3xFLAG, BFP, HIV NES, and T2A

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_TNFAIP3_WT

Plasmid

#82238

Purpose

Gateway Donor vector containing TNFAIP3 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TNFAIP3 (TNFAIP3 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-Nr4a1 double gRNAs

Plasmid

#162791

Purpose

Multiplex Guide RNAs to generate Nr4a1 knockout by CRISPR

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Multiplex Nr4a1 gRNA 1 and 2 (Nr4a1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAC1_p.T17N

Plasmid

#82179

Purpose

Gateway Donor vector containing RAC1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RAC1 (RAC1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

T17N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-SCGB3A2 promoter-EGFP-EF1a-TagRFP

Plasmid

#204821

Purpose

Lentiviral vector for human SCGB3A2 promoter-driven expression of EGFP

Depositor

Emma Rawlins

Article

He et al Cell. 2022 Dec 8;185(25):4841-4860.e25.

Insert

An upstream region including human SCGB3A2 promoter

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SCGB3A2 promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_SOCS3_WT

Plasmid

#82245

Purpose

Gateway Donor vector containing SOCS3 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SOCS3 (SOCS3 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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V5-ERG-R367K

Plasmid

#118624

Purpose

Expresses V5 tagged ERG R367K (DNA binding mutant) protein

Depositor

William Hahn

Article

Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

Insert

ERG (R367K DNA binding mutant) (ERG Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

R367K

Promoter

EF1a

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mM-Asn209Asp_M18

Plasmid

#91781

Purpose

Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Asp (EU numbering).

Depositor

Joanna Bereta

Article

Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

Insert

immunoglobulin heavy constant mu (Ighm Mouse)

Use

Tags

Expression

Mammalian

Mutation

Asn209 changed to Asp (EU numbering)

Promoter

hEF1-HTLV prom

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CNTF-HA

Plasmid

#195517

Purpose

Expresses HA-tagged CNTF in mammalian cells

Depositor

Kevin Park

Article

Ribeiro et al Exp Neurol. 2022 Sep;355:114147. doi: 10

Insert

Ciliary neurotrophic factor (CNTF Human)

Use

AAV

Tags

HA-tag and NGF signal peptide

Expression

Mammalian

Mutation

Promoter

UbC promoter with beta-globin intron

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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PB-mCherry-DMDEx23-eGFP

Plasmid

#211366

Purpose

Piggybac transposon plasmid for a DMD exon 23 skipping reporter (mCherry-DMDEx23)

Depositor

Ulrich Lächelt

Article

Lessl et al Bioconjug Chem. 2023 Dec 20;34(12):2263-

Insert

mCherry-DMDEx23-eGFP

Use

Tags

Expression

Mammalian

Mutation

mCherry interrupted by mdx dystrophin exon 23 bet…

Promoter

CAG

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NRAS_p.Q61L

Plasmid

#81674

Purpose

Gateway Donor vector containing NRAS, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NRAS (NRAS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Q61L

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_POT1_p.G76V

Plasmid

#81497

Purpose

Gateway Donor vector containing POT1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

POT1 (POT1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G76V

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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p1.1-mCherry

Plasmid

#162772

Purpose

Fluorescent reporter for CHO expression studies

Depositor

Ivan Vorobiev

Article

Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

Insert

red fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

consensus Kozak sequence (GCCGCCATGG) added befor…

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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p1.2-GS-eGFP

Plasmid

#162771

Purpose

Fluorescent reporter for CHO expression studies

Depositor

Ivan Vorobiev

Article

Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

Insert

enhanced green fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

consensus Kozak sequence (GCCGCCATGG) added befor…

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_POT1_p.L388M

Plasmid

#81570

Purpose

Gateway Donor vector containing POT1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

POT1 (POT1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

L388M

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_POT1_p.L265H

Plasmid

#81559

Purpose

Gateway Donor vector containing POT1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

POT1 (POT1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

L265H

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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