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Showing: 8421 - 8440 of 20484 results

  3. pYPQ136-tRNA2.0

    Plasmid
    #158398
    Purpose
    Golden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158398-plasmid-map-sequence-id-330906

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  5. pPBO.ACT006

    Plasmid
    #182716
    Purpose
    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning
    Depositor
    Joseph Schoeniger
    Article
    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666
    Insert
    CasRx crRNA cloning backbone
    Use
    CRISPR
    Tags
    GFP in cloning site of crRNA for easier screening…
    Expression
    Bacterial
    Mutation
    Catalytically deactivated R295A, H300A, R849A, H8…
    Promoter
    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)
    Available since
    Aug. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    182716-plasmid-map-sequence-id-361297

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  10. pLib6.4B

    Plasmid
    #176665
    Purpose
    Expression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism
    Depositor
    Norbert Perrimon
    Article
    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:
    Type
    Empty backbone
    Use
    Crispr
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176665-plasmid-map-sequence-id-348636

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  13. pSL0747 (pDonor_L-MmeI)

    Plasmid
    #130647
    Purpose
    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchCAST donor DNA (L*)
    Use
    CRISPR; Transposon
    Tags
    Expression
    Bacterial
    Mutation
    TGTTGATG --> TGTTGGAG
    Promoter
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130647-plasmid-map-sequence-id-255647

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  16. pLib6.6

    Plasmid
    #176652
    Purpose
    Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism
    Depositor
    Norbert Perrimon
    Article
    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:
    Type
    Empty backbone
    Use
    Crispr
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176652-plasmid-map-sequence-id-348545

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  20. pSL0746 (pDonor_R-MmeI)

    Plasmid
    #130646
    Purpose
    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchCAST donor DNA (R*)
    Use
    CRISPR; Transposon
    Tags
    Expression
    Bacterial
    Mutation
    TGTTGATA --> TGTTGGAA
    Promoter
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130646-plasmid-map-sequence-id-255642

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Showing: 8421 - 8440 of 20484 results