-
Plasmid#103147PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7b-3p target (MIRLET7B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7d-5p
Plasmid#103151PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7d-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7d-5p target (MIRLET7D Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
KAT6B-luciferase
Plasmid#113353Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B geneDepositorInsertKAT6B-enhancer (KAT6B Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {hCAR}off-{ChETA}on-WPRE
Plasmid#111388PurposeAAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)DepositorInsertsUseAAVTagsHA and MycExpressionMammalianMutationPromoterhSynapsinAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
RV-cag-Dio-Kif5c-2A-tdt
Plasmid#87668Purposeretroviral vector, conditional expression membrane Tdtomato and Kif5Cdelta560DepositorInsertKif5C-P2A-mTdt (Kif5c Rat)
UseRetroviralTagspalmitylationExpressionMutationmotor domain aa1-560 present, V40A, E125V (see de…PromoterCAGAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-owlTRIMCyp-FOS K283D Q287D
Plasmid#79068PurposeExpress owl monkey TRIMCyp K283D, Q287D in mammalan cellsDepositorInsertTRIM5Cyp (owl monkey) (K283D, Q287D)-FOS
UseTagsOneSTrEP-FLAGExpressionMammalianMutationChanged Lys 283 to Asp and Gln 287 to AspPromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
Plasmid#79030PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
UseTagsOneSTrEP-FLAGExpressionInsectMutationdeleted amino acid 294-515 from frican green monk…PromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (205-222) L217A (pGEX2T(TEV))
Plasmid#80638PurposeResidues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-204DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 205-222; Changed Leucine 217 to AlaninePromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (205-222) W220A (pGEX2T(TEV))
Plasmid#80637PurposeResidues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-203DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 205-222; Changed Trp 220 to AlaPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (205-222) L214A (pGEX2T(TEV))
Plasmid#80639PurposeResidues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-205DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 205-222; Changed Leucine 214 to AlaninePromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3XFLAG-T34A-GATA6-3XAU1 puro
Plasmid#72609PurposeFor mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single Threonine#34 to alanine mutationDepositorInsertGATA binding protein 6 (GATA6 Human)
UseRetroviralTags3XAU1 and 3XFLAGExpressionMammalianMutationchanged threonine34 to alaninePromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3XFLAG-S33A-GATA6-3XAU1 puro
Plasmid#72610PurposeFor mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#33 to alanine mutationDepositorInsertGATA binding protein 6 (GATA6 Human)
UseRetroviralTags3XAU1 and 3XFLAGExpressionMammalianMutationchanged serine33 to alaninePromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3XFLAG-S37A-GATA6-3XAU1
Plasmid#72613PurposeGateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged S37A mutant human GATA6DepositorInsertGATA binding protein 6 (GATA6 Human)
UseEntry vector for gateway cloningTags3XAU1 and 3XFLAGExpressionBacterialMutationChanged serine37 to alaninePromoterTRE tight promoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
mOC-STAMP (N162D)_3xFlag-Myc-CMV26
Plasmid#73578PurposeExpress mouse OC-STAMP ((N162D) in mammalian cellsDepositorInsertOC-STAMP (Ocstamp Mouse)
UseTags3x Flag and c-Myc tagExpressionMammalianMutationchanged asparagin (N) 162 to Aspartic Acid (D)PromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
MT583
Plasmid#63114PurposeThe gDNA construct is designed for arabidopsis ecotype Est-1 ACD6 expression in other genetic backgrounds.DepositorInsertACD6 (Accelerated Cell Death 6)
UseTagsExpressionPlantMutationPromoterAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-IRES-miR34a-P6
Plasmid#64790PurposeLuciferase expression driven by miR-34a promoter P6DepositorInsertmiR-34a promoter P6 (MIR34A Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-IRES-miR34a-P3
Plasmid#64787PurposeLuciferase expression driven by miR-34a promoter P3DepositorInsertmiR-34a promoter P3 (MIR34A Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-IRES-miR34a-P5
Plasmid#64789PurposeLuciferase expression driven by miR-34a promoter P5DepositorInsertmiR-34a promoter P5 (MIR34A Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-IRES-miR34a-P4
Plasmid#64788PurposeLuciferase expression driven by miR-34a promoter P4DepositorInsertmiR-34a promoter P4 (MIR34A Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Report-Luc-KLF4-K2S
Plasmid#62980PurposeTranslational reporter for KLF4DepositorInsertKLF4 partial sequence (KLF4 Human)
UseLuciferaseTagsfirefly luciferaseExpressionMutationFragment 446-911 of KLF4PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only