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pNP1 OR sfGFP

Plasmid

#107364

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 OR input A and pNP1 OR input B are co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

OR gate + sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0816

Plasmid

#133510

Purpose

Expression of mTagBFP2 tagged CRIB domain to monitor active Cdc42

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(act1)-CRIB(gic2aa2-181)-mTagBFP2-terminator(ScADH1)-bsdMX

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pUCBB-ctH6-eGFP

Plasmid

#32562

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

6 His tag with Thrombin site

Expression

Mutation

Promoter

Consitutive lac

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pRRG159-foxD

Plasmid

#99068

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea foxD

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

foxD in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pRPB1-CTD14

Plasmid

#112822

Purpose

pSMF2 with 14 CTD repeats

Depositor

Stephen Fuchs

Article

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

CTD repeats have identical sequence, deleted repe…

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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mEos2-Calreticulin-N-10

Plasmid

#57353

Purpose

Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Calreticulin (CALR Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAB031a

Plasmid

#79221

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ HA4-Zif268; PlacIq lacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ; PlacIq

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAG416GPD-Cerulean-CHC1

Plasmid

#18850

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

CHC1 (CHC1 Budding Yeast)

Use

Tags

Cerulean

Expression

Yeast

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-5X-1-DJ1-M26I

Plasmid

#29353

Depositor

Mark Cookson

Article

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Met26Ile

Promoter

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pMOD_C4300

Plasmid

#91103

Purpose

Module C, Promoter: none, Gene: BaeI LacZ cassette for GT donor cloning, Terminator: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

BaeI LacZ cassette for GT donor cloning

Use

Unspecified

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pNL1 Ecarin

Plasmid

#106289

Purpose

Expresses Ecarin in Ecoli off a T7 promoter in the NEB control cloning vector

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

Ecarin

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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CRY2(delta2-6)-mCherry

Plasmid

#104182

Purpose

Expresses fusion of CRY2(delta aa 2-6) with mCherry

Depositor

Bianxiao Cui

Article

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2(delta2-6)-mCherry

Use

Tags

Expression

Mammalian

Mutation

CRY2PHR with deletion of aa 2-6

Promoter

CMV

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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CRY2(neutral2-6)-mCherry

Plasmid

#104181

Purpose

Expresses fusion of CRY2(neutral aa 2-6) with mCherry

Depositor

Bianxiao Cui

Article

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2(neutral2-6)-mCherry

Use

Tags

Expression

Mammalian

Mutation

CRY2PHR K2S M3S D4G K5S K6S

Promoter

CMV

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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RhoJ

Plasmid

#55696

Purpose

expression of mouse RhoJ

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoJ (Rhoj Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pSG 413

Plasmid

#63859

Purpose

Detect translation initiation from CUG start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

CUG start codon

Promoter

PtacI

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
FusionRed-CytERM-N-17

Plasmid

#56110

Purpose

Localization: Endoplasmic Reticulum, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

CytERM

Use

Tags

FusionRed

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
EGFP-bSV-171-1792

Plasmid

#41661

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

amino acids 171-1792

Promoter

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
pMOD_A0202

Plasmid

#91003

Purpose

Module A, Promoter: AtUbi10, Gene: AtCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A nickase

Use

CRISPR

Tags

Expression

Mutation

D10A

Promoter

AtUbi10

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-his-3C_Clu3B6p

Plasmid

#100093

Purpose

expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide

Depositor

Nikolai Sluchanko

Article

Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10

Insert

14-3-3 sigma human (SFN Human)

Use

Tags

HSPB6 peptide and His6

Expression

Bacterial

Mutation

75-77 residues changed to AAA to facilitate cryst…

Promoter

T7

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-bSV-831-1281

Plasmid

#40962

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-830 and 1282-1792

Promoter

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only