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Plasmid#176743PurposeExpresses Connexin-43 fused with BioID2 at the C-terminus in astrocytesDepositorInsertCx43-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pdRBFC-NS1-YC
Plasmid#74724PurposeExpress C-terminal YC fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labelingDepositorInsertInfluenza A virus NS1 protein, partial
UseDouble-stranded rna labelingTagsFLAG and YFP C-terminal half domainExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS L30 Ruby3-Rab6A
Plasmid#135653PurposeRab6A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.DepositorInsertRab6A
UseTagsRuby3ExpressionMammalianMutationPromoterL30Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSR358.4
Plasmid#125114PurposeExpression of narL(1-159aa)-liaR(154-211aa) chimera under PLtetO-1, output Promoter PliaI121DepositorInsertsnarL(REC)-liaR(DBD)159
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV10-hTel2
Plasmid#30214DepositorInserttelomere maintenance 2 (TELO2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSYN-eGFP
Plasmid#162512PurposeAAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (hSYN) promoterDepositorInserteGFP
UseAAVTagsExpressionMammalianMutationPromoterhSYNAvailable sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC001 - huLshC2C2-MBP for bacterial expression
Plasmid#79150PurposeExpresses human codon-optimized LshC2c2 for purification in E. coliDepositorInsertC2c2
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-Turbo-L2
Plasmid#127350Purposegateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteinsDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, V5, and YFPExpressionMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Ash2L full
Plasmid#15548DepositorInsertAsh2L (ASH2L Human)
UseTagsExpressionMammalianMutationR622H (Please see depositor comments below)PromoterAvailable sinceAug. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
CMV-T7-hOsa2
Plasmid#17987DepositorInsertOsa2 (ARID1B Human)
UseTagsT7 epitopeExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28a SUMO Nup53xl 1-320
Plasmid#85998Purposebacterial expression of his6 and SUMO tagged Xenopus laevis Nup53DepositorInsertXenopus laevis Nup53 (nup35.L Frog)
UseTagsSUMOExpressionBacterialMutationcodon optimzed for bacterial expressionPromoterT7Available sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET-4xTR-TUBE
Plasmid#110312PurposeE. coli expression and purification of 4xTR-TUBEDepositorInsert4xTR-TUBE (UBQLN1 Human)
UseTagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTS100 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-TEV-MCS
Plasmid#199353PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-TEV-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTagBFP-P2A-ALFA-TEVExpressionMammalianMutationWTPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti6_YAP1-V5
Plasmid#173002Purposeconstitutive expression of V5-tagged YAP1DepositorInsertYAP1-V5 (YAP1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABE-LynG
Plasmid#206867PurposeExpression of LynG probe - LynAnchor-eGFPDepositorArticleInsertLynG
UseRetroviralTagseGFPExpressionMammalianMutationContains Lyn amino acids 1-16PromoterAvailable sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-Turbo-NES-L2
Plasmid#127349Purposegateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteinsDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPExpressionMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTS113 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-Alfa-P2A-TagBFP
Plasmid#199366PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTags3C-SUMOEu-ALFA-P2A-TagBFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
ATGL*-V5-APEX2
Plasmid#170574PurposeA C-terminal APEX2 fusion to the human ATGL S47A proteinDepositorInsertATGL* (PNPLA2 Human)
UseLentiviralTagsV5-APEX2ExpressionMammalianMutationchanged serine 47 to alaninePromoterCMVAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-Cas9GFP-W
Plasmid#200100PurposeLentiviral vector expressing Cas9 fused with GFP at the C-terminusDepositorInsertGFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)10-(SIM)5
Plasmid#126866PurposeBacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.DepositorInsert10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only