Addgene

Showing: 8381 - 8400 of 22772 results

Results per page:

Sort results by:

pHAGE-SSFV-dCas9-GFP

Plasmid

#64105

Purpose

Expression of Sp dCas9-GFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

SSFV

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pPLN-hFluc-DM-eGFP-KDEL

Plasmid

#177728

Purpose

DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

hFlucDM

Use

Tags

KDEL, Prolactin Signal Sequence, and eGFP

Expression

Bacterial and Mammalian

Mutation

R188Q, R261Q (Destabilized Mutant)

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-PD-1 (Y223F, Y248F)-mGFP

Plasmid

#180790

Purpose

Expression of human PD-1 (Y223F, Y248F) fused to mGFP

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

PD-1 (Y223F, Y248F) (PDCD1 Human)

Use

Lentiviral

Tags

Expression

Mutation

PD-1 (Y223F, Y248F)

Promoter

SFFV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yEPAGFP-CaUra3

Plasmid

#44649

Depositor

Kurt Thorn

Insert

yEPAGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
peAAV-CKII(0.4)-Chronos_eGFP_GP2A_nls_mScarlet

Plasmid

#121542

Purpose

Chronos with red nucleus-targeted fluorescence for excitatory neurons

Depositor

Matthias Prigge

Insert

blue-absorbing channelrhodopsin Chronos with eGFP and nls-mScarlet

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

CamKII

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE-EGFP-FLAG-TK1

Plasmid

#161919

Purpose

Expresses human TK1 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

Thymidine kinase, cytosolic (TK1 Human)

Use

Tags

GFP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCasper-pNos-PCP-eGFP

Plasmid

#180274

Purpose

to epress PCP-GFP fusion protein in the early fly embryos (driven by maternal nanos driver)

Depositor

Hernan Garcia

Article

Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

Insert

PCP-eGFP

Use

Tags

eGFP

Expression

Insect

Mutation

Promoter

nanos

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHBS1413 GFP-TDP43 VLIM-F

Plasmid

#118802

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mutation

All VLIM to F in CTD of full-length TDP43

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSFG-TFII-I-GFP beta

Plasmid

#22200

Depositor

Ananda Roy

Article

Hakre et al Mol Cell. 2006 Oct 20. 24(2):301-8.

Insert

TFII-I (GTF2I Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pET23-CBD-(-PP)-EGFP-H246,249D

Plasmid

#12598

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Type

Empty backbone

Use

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
GFP-PCAF (delta5-53a.a.)

Plasmid

#65388

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

PCAF (KAT2B Human)

Use

Tags

EmGFP and V5

Expression

Mammalian

Mutation

lost 13-159bp of the ORF

Promoter

CMV

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only
pQL47 EGFP-VAMP-GST

Plasmid

#122971

Purpose

Reporter of botulinum toxin B cleavage

Depositor

Chandra Tucker

Article

Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

Insert

EGFP-VAMP3-GST

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-KSENB-Nup133-mut

Plasmid

#87339

Purpose

To express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP133 (NUP133 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes; deletion of Nup133 AA1-7

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKC518 pBABE EGFP-LIN52

Plasmid

#192697

Purpose

Retroviral transgene expression of human LIN52

Depositor

Iain Cheeseman

Article

Funk et al Cell. 2022 Nov 23;185(24):4634-4653.e22.

Insert

LIN52 (LIN52 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
bdSUMO-pHD118-GFP-HiBit

Plasmid

#171015

Purpose

For bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-pHD118-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GST(LSCS)GFP-ActA

Plasmid

#104452

Purpose

Mitophagy cargo assay

Depositor

Carol Mercer

Article

Dennis et al Methods Enzymol. 2009;452:97-118. doi: 1

Insert

GST-GFP-ActA

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-kanMX6-P81nmt1-GFP

Plasmid

#39291

Depositor

John Pringle, Jurg Bahler

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

-1163 to +6 of nmt1 locus with a 7bp deletion (AT…

Promoter

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
pHBS1411 GFP-TDP43 FYW-S

Plasmid

#118800

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mutation

All FYW to S in CTD of full-length TDP43

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTTH+eGFP-bromo5(N140A)

Plasmid

#157806

Purpose

Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1 with N140A mutation

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

eGFP-bromo5(N140A)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

N140A

Promoter

pTAC

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES Centrin1 GFP Pur

Plasmid

#64341

Purpose

Bicistronic expression of Centrin-GFP fusion and Puro resistance gene

Depositor

Matthieu Piel

Insert

Centrin1 (CETN1 Human)

Use

Tags

GFP and IRES-Puro

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only