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mOC-STAMP (N162D)_3xFlag-Myc-CMV26

Plasmid

#73578

Purpose

Express mouse OC-STAMP ((N162D) in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

Insert

OC-STAMP (Ocstamp Mouse)

Use

Tags

3x Flag and c-Myc tag

Expression

Mammalian

Mutation

changed asparagin (N) 162 to Aspartic Acid (D)

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pRFP shRNA-resistant TrkB F616A -S478A

Plasmid

#72718

Purpose

Mammalian expression of TrkB fused to RFP in C-terminus, shRNA resistant and lacking TIAM1-binding site

Depositor

Freddy Jeanneteau

Article

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

TrkB

Use

Tags

RFP

Expression

Mammalian

Mutation

shRNA-resistant TrkB (T300C, T301A, C302G, C306A,…

Promoter

CMV

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only
Anti-Ankyrin-G (staining) [N106/36R-rat IgG2a]

Plasmid

#225416

Purpose

Mammalian Expression Plasmid of anti-Ankyrin-G (staining) (Human) rat IgG2a R-mAb. Derived from hybridoma N106/36.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Ankyrin-G (staining) (Homo sapiens) recombinant (Mouse) monoclonal antibody. (ANK3 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1

Plasmid

#107407

Purpose

Lentiviral expression of gRNA resistant PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

sg resistant PBRM1 (PBRM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

sgRNA resistant

Promoter

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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Mammalian expression: Z-lock cofilin dark state mutant

Plasmid

#141136

Purpose

a control for optogenetic cofilin analog

Depositor

Klaus Hahn

Article

Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

Insert

Zdk2, Cofilin, LOV2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4 h-Stx3 resistant to shRNA #304-2xMyc/His

Plasmid

#99746

Purpose

Expresses human Stx3 with a C-term myc-myc-his that is resistant to shRNA #304 from Sigma Aldrich.

Depositor

Thomas Weimbs

Article

Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

Insert

Stx3 (STX3 Human)

Use

Tags

myc-myc-his

Expression

Mammalian

Mutation

Made six (6) silent mutations to confer resistanc…

Promoter

CMV/TO

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTS97 pHAGE2 CMVtetO2 GFP-22xGS-SUMOstar-MCS

Plasmid

#199350

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOstar-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP-SUMOstar

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS108 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-GFP

Plasmid

#199361

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-SUMOstar-EGFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Chx10 (2.5kb distal 5' reg)+SV40p-tdTomato-IRES-AP

Plasmid

#18814

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

Chx10 (2.5kb distal 5' reg)+SV40p-tdTomato-IRES-AP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
JI401: Ad5 E1A/Hexon qRT-PCR standard

Plasmid

#131753

Purpose

Plasmid standard containing regions of the Ad5 E1A and Ad5 Hexon genes to create a qRT-PCR standard curve for detection of replication competent adenovirus (RCA).

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Inserts

Ad5 E1A gene fragment
Ad5 Hexon gene fragment

Use

Standard for qrt-pcr

Tags

Expression

Mutation

Promoter

N/A

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pALPS_3'LTR GFP@gag start SFFV rev

Plasmid

#101339

Purpose

LTR drives GFP and internal SFFV promoter drives codon optimized rev

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

GFP, rev

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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E. coli expression: Z-lock cofilin dark state mutant

Plasmid

#141135

Purpose

a control for optogenetic cofilin analog

Depositor

Klaus Hahn

Article

Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

Insert

Zdk2, Cofilin, LOV2

Use

Tags

Expression

Bacterial

Mutation

D3 deletion, I32F in Zdk2

Promoter

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pALPS_3'LTR GFP@gag start SFFV tat

Plasmid

#101338

Purpose

LTR drives GFP and internal SFFV promoter drives codon optimized tat

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

GFP, tat

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG

Plasmid

#200792

Purpose

Mammalian expression of CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Use

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

Δ954-1027

Promoter

CMV & T7

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

Plasmid

#200798

Purpose

Mammalian expression of CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Use

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

411-680

Promoter

CMV & T7

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-TRIP8b (constant) ion channel [N212/27R]

Plasmid

#220409

Purpose

Mammalian Expression Plasmid of anti-TRIP8b (constant) ion channel (Rat) IgG2a R-mAb. Derived from hybridoma N212/27.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-TRIP8b (constant) ion channel (Rattus novegicus) recombinant mouse monoclonal antibody. (Pex5l Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pRDAX-609 C-Tag Venus210[2] L20 Stat3 mCherry

Plasmid

#130084

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3 (Stat3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-610 N-Tag Venus210[2] L20 Stat3 mCherry

Plasmid

#130085

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3 (Stat3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-612 N-Tag Venus210[2] L32 Stat3 mCherry

Plasmid

#130087

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3 (Stat3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-554 N-Tag GFP[1] L10 Stat3 EBPF2

Plasmid

#130101

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3 (Stat3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge