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MTK234_056

Plasmid

#123921

Purpose

Encodes the spCas9 gRNA GFP dropout expression cassette (human u6 promoter and constant region 3) as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

gRNA-GFPDROPOUT-hu6_20N_cr3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_016

Plasmid

#123931

Purpose

Encodes the phiC31 attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Phic31attB tagBFP destination vector

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_026

Plasmid

#123906

Purpose

Encodes the spCas9 human non-targeting gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

human non targeting sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_028

Plasmid

#123908

Purpose

Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA2 -hThal5.10-2_hg18

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_029

Plasmid

#123909

Purpose

Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA3 -hThal5.10-2_hg18

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK6_013

Plasmid

#123867

Purpose

Encodes Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) expressing a Blasticidin resistance cassette without a terminator to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

WPRE-Blast (no terminator)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3_018

Plasmid

#123733

Purpose

Encodes rapamycin and tamoxifen activated split-dCAS9 (N-terminal) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

spdCas9(1-204)-frb-D10A-ERT2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pHES 946

Plasmid

#112644

Purpose

blue light inducible cryptochrome based optogenetic expression system

Depositor

Hana El-Samad

Article

Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

Insert

RTG3AD-GSx7-CIB1-SV40NLS (CIB1 Mustard Weed, Budding Yeast, Synthetic)

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57.1-MAT2A

Plasmid

#100521

Purpose

Dox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

MAT2A (MAT2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

pTight TRE

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMax_mOrange_G299C

Plasmid

#177829

Purpose

Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).

Depositor

Leona Samson, Zachary Nagel

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mOrange_G299C

Use

Tags

Expression

Mammalian

Mutation

G299C

Promoter

CMV

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMax_mOrange_A215C

Plasmid

#177828

Purpose

Site-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).

Depositor

Leona Samson, Zachary Nagel

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mOrange_A215C

Use

Tags

Expression

Mammalian

Mutation

A215C

Promoter

CMV

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMax_BFP_A191G

Plasmid

#177823

Purpose

Site-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).

Depositor

Leona Samson, Zachary Nagel

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

tagBFP_A191G

Use

Tags

Expression

Mammalian

Mutation

A191G

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pMax_GFP_C289T

Plasmid

#177826

Purpose

Site-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).

Depositor

Leona Samson, Zachary Nagel

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

GFP_C289T

Use

Tags

Expression

Mammalian

Mutation

C289T

Promoter

CMV

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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PIK3CA - Exon20 H1047R

Plasmid

#16639

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA H1047R (PIK3CA Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

H1047R

Promoter

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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OmEF1aCas9P2ApuroSB

Plasmid

#165484

Purpose

Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells

Depositor

Dietmar Kueltz

Article

Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10

Insert

Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)

Use

CRISPR; Transposon

Tags

Expression

Mutation

Promoter

Oreochromis mossambicus EF1 alpha promoter (OmEF1…

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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CD40L-MIEG-hCD4 (hCD4-CD40L)

Plasmid

#40355

Depositor

Alexander Dent

Article

Kusam et al Int J Cancer. 2009 Aug 15;125(4):977-81.

Insert

CD40L (Cd40lg Mouse)

Use

Retroviral

Tags

IRES hCD4

Expression

Mutation

Promoter

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-ERBB4 (kinase dead)

Plasmid

#29533

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Tags

Expression

Mammalian

Mutation

changed lysine 751 to methionine

Promoter

Available since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-ERBB4 (kinase dead)

Plasmid

#29544

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

changed lysine 751 to methionine

Promoter

Available since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
JPF0419d

Plasmid

#124020

Purpose

Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pCAG-Lyn-iRFP713-P2A-NES-mAzamiGreen-P2A-p38KTR-mCerulean-P2A-H2B::mScarlet

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4A-MITF-M (D380N)

Plasmid

#31248

Depositor

Yardena Samuels

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

MITF-M (MITF Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Aspartic acid 380 changed to Asparagine

Promoter

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only