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Plasmid#91821PurposeAID CT-tag linker donor for the SapTrap cloning systemDepositorInsertAuxin-inducible degron (AID)
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_Psyn-sgRNA500
Plasmid#149613PurposegRNA expression in B. burgdorferi, parental vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJUMP29-1A(sfGFP)
Plasmid#126960PurposeLevel 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDD364
Plasmid#91831PurposeLox511I-flanked SEC donor for the SapTrap cloning systemDepositorInsertLox511I-SEC-Lox511I
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
MXS_PGK
Plasmid#62414PurposeMXS_chaining vector with PGKDepositorInsertPGK Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMEXC3GH (Apr)
Plasmid#110083PurposeAnalogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-34
Plasmid#119998PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with 3xFLAGDepositorInsert3xFLAG
UsePcr templateTags3xFLAGExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDD382
Plasmid#91830PurposeLox2272-flanked SEC donor for the SapTrap cloning systemDepositorInsertLox2272-SEC-Lox2272
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJUMP19-Ptac_P
Plasmid#127004PurposeBasic Part P- Promoter (non-constitutive); Ptac; IPTG/lactose induced; controlled by LacI.DepositorInsertPart Ptac_P
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEC578
Plasmid#174366PurposedCas9 for modular CRISPRa. SYNZIP-dCas9. Expresses dCas9 with a SYNZIP18 domain fused to the N terminus.DepositorInsertdCas9 fused to SYNZIP18
UseTagsExpressionBacterialMutationPromoterJ23150Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZLSLD.R
Plasmid#51511PurposeSingle component LSL vector. Contains 2x35S:Zif268:FokI outside of the replicon borders. Within the replicon is the us:NPTII template.DepositorInsertsZif268:FokI
us:NPTII repair template
UsePlant t-dna plasmidTagsNLSExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PflaBS-sgRNA500
Plasmid#149615PurposegRNA expression in B. burgdorferi, parental vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaBSAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_P0026-sgRNA500
Plasmid#149620PurposegRNA expression in B. burgdorferi, parental vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterP0026Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDD375
Plasmid#91825PurposeCodon-optimized mKate2 donor for the SapTrap cloning systemDepositorInsertmKate2-C1
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-01
Plasmid#119980PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with moxBFPDepositorInsertmoxBFP
UsePcr templateTagsmoxBFPExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-28
Plasmid#120007PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with 3xHADepositorInsert3xHA
UsePcr templateTags3xHAExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP49-2E(sfGFP)
Plasmid#126995PurposeLevel 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
MXS_Tet
Plasmid#62422PurposeMXS_chaining vector with TetDepositorInsertTetracycline inducible promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
MXS_tTA2
Plasmid#62429PurposeMXS_chaining vector with rTA2DepositorInsertTetracycline transactivator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDD397
Plasmid#91822PurposeAID FP-slot donor for the SapTrap cloning systemDepositorInsertAuxin-inducible degron (AID)
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only