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Plasmid#160218PurposeAtpU6-26 Golden Gate level 0 piece to express gRNAsDepositorInsertpAtU6-26 promoter
UseGolden gate level 0 piece to express grnasTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-AtpU6-29
Plasmid#160219PurposeAtpU6-29 Golden Gate level 0 piece to express gRNAsDepositorInsertpAtU6-29 promoter
UseGolden gate level 0 piece to express grnasTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Human_TFAM_HMGA_pET28
Plasmid#167326PurposeExpresses TFAM HMGA mutant in bacterial cellsDepositorInsertTFAM (TFAM Human)
UseTags6XHisExpressionBacterialMutationTruncation mutant containing HMGA domain (43-122)PromoterT7Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human_TFAM_HMGA_linker_pET28
Plasmid#167327PurposeExpresses TFAM HMGA+linker mutant in bacterial cellsDepositorInsertTFAM (TFAM Human)
UseTags6XHisExpressionBacterialMutationTruncation mutant containing HMGA and linker doma…PromoterT7Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJIT163-AFID-2
Plasmid#167358PurposeFor inducing predictable deletions in protoplasts and wheat plantsDepositorInsertA3A-Cas9-UDG
UseTagsExpressionPlantMutationWTPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSC1
Plasmid#91179PurposeT-DNA vector expressing TALENs targeting Medtr4g020620 and Medtr2g013650ADepositorInsertTALENs targeting Medtr4g020620 and Medtr2g013650A
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_38J
Plasmid#91154PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:npt IIDepositorInsertEngineering Reagent: 35S:TALEN
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A4010
Plasmid#91055PurposeModule A, Promoter: ZmUbi, Gene: dTaCas9-VP64, Terminator: HSPDepositorInsertdTaCas9-VP64
UseCRISPRTagsExpressionMutationPromoterZmUbiAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A4210
Plasmid#91057PurposeModule A, Promoter: Zmubi, Gene: Csy4-P2A-dTaCas9-VP64, Terminator: HSPDepositorInsertCsy4-P2A-dTaCas9-VP64
UseCRISPRTagsExpressionMutationPromoterZmubiAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A3801
Plasmid#91053PurposeModule A, Promoter: 35S, Gene: Csy4-P2A-dAtCas9-VP64, Terminator: HSPDepositorInsertCsy4-P2A-dAtCas9-VP64
UseCRISPRTagsExpressionMutationPromoter35SAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_39J
Plasmid#91155PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:barDepositorInsertEngineering Reagent: 35S:TALEN
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0302
Plasmid#91006PurposeModule A, Promoter: AtUbi10, Gene: AtCas9_H840A nickase, Terminator: HSPDepositorInsertAtCas9_H840A nickase
UseCRISPRTagsExpressionMutationH840APromoterAtUbi10Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0308
Plasmid#91007PurposeModule A, Promoter: EC1.2, Gene: AtCas9_H840A nickase, Terminator: HSPDepositorInsertAtCas9_H840A nickase
UseCRISPRTagsExpressionMutationH840APromoterEC1.2Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXY029 (mEos2-MTSBs)
Plasmid#72652PurposeInducible expression of mEos2-MTSBs in bacteriaDepositorInsertmEos2-MTSBs
UseTagsmEos2ExpressionBacterialMutationPromoterT5-lacAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabePuro-DNAJA4CDS
Plasmid#43799DepositorInsertDNAJA4 CDS (DNAJA4 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterSV40Available sinceMarch 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
Nb-gp41-GFP (MoonTag-Nb-GFP)
Plasmid#128602PurposeExpresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).DepositorInsertNanobody_gp41
UseLentiviralTagsGB1 and sfGFPExpressionMutationPromoterSFFVAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_iRFP670 sgAAVS1
Plasmid#192477PurposeAll-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a controlDepositorInsertSpCas9
UseTagsT2A iRFP670ExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_mCherry sg1-sg2
Plasmid#192479PurposeAll-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1DepositorInsertSpCas9
UseTagsT2A mCherryExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-5'-ENGRAM
Plasmid#179157PurposepiggyBac plasmid for 5' ENGRAM recorderDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterminPAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nb-gp41-Halo (MoonTag-Nb-Halo)
Plasmid#128603PurposeExpresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).DepositorInsertNanobody_gp41
UseLentiviralTagsGB1 and HaloExpressionMutationPromoterSFFVAvailable sinceAug. 12, 2019AvailabilityAcademic Institutions and Nonprofits only