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pEGFP eIF2beta C-term (aa 89-331)
Plasmid#45901DepositorInserteIF2S2 aa89-331 (Eif2s2 Mouse)
UseTagsEGFPExpressionMammalianMutationaa 89-331PromoterCMVAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-H136T
Plasmid#58413PurposeExpresses murine IFITM3-H136T with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)DepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationHistidine 236 to ThreoninePromoterCMV IEAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSV HA eIF2beta (Pro110-113)
Plasmid#45899DepositorInserteIF2beta (Eif2s2 Mouse)
UseTagsHAExpressionMammalianMutationP110A, P113APromoterSV40Available sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGME
Plasmid#71252Purposeluciferase reporter with GM-CSF promoter + 717 bp Bgl II GM-CSF enhancerDepositorInsertGM-CSF enhancer (CSF2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterGM-CSFAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-EGFP-CLASP2 340-1362
Plasmid#24522DepositorInsertCLASP2 (340-1362) (CLASP2 Human)
UseAdenoviralTagsEGFPExpressionMammalianMutationCLASP2 deletion mutant that retains MT lattice bi…PromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
DNA-PKcs PQR>ala
Plasmid#83319Purposehuman DNA-PKcs PQR>alaDepositorInserthuman DNA-PKcs (PRKDC Human)
UseTagsExpressionMammalianMutation5 phosphorylation sites in the PQR cluster substi…PromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-SynTetOff-myrGFP
Plasmid#190233PurposeAAV vector, high-level expression of EGFP with a myristoylation/palmitoylation signal derived from Fyn N-terminus (myrGFP) in neuronal cellsDepositorInsertEGFP
UseAAVTagsmyristoylation signal sequenceExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLPCX-Cx43-IRES-GFP
Plasmid#65433PurposeMammalian expression of Cx43 and EGFP seperately driven by the ECMV IRES. For transfection or retroviral productionDepositorInsertCx43 (GJA1 Human)
UseRetroviralTagsIRES-GFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.4RT4-Pct5.1-crRNA(cgr2)-RT(Δcgr12)
Plasmid#191650PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cgr2-targeting spacer) cgr1/cgr2-deleting repair template, used for cgr1/cgr2 deletionDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk1a1(apf)
Plasmid#13846DepositorInsertJnk1a1(apf) (MAPK8 Human)
UseTagsFlagExpressionMammalianMutationreplaced dual phosporylation motif Thr(183)-Pro-T…PromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
Stat3 S727A pRc/CMV
Plasmid#8708DepositorInsertStat3 S727A (Stat3 Mouse)
UseTagsExpressionMammalianMutationS727A (exhibits o serine phosphorylation either c…PromoterAvailable sinceAug. 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
psigma2-EGFP
Plasmid#53610PurposeExpresses sigma2 of adaptor protein of AP2DepositorInsertsigma2 (Ap2s1 Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGM
Plasmid#71250Purposeluciferase reporter with human GM-CSF promoter -627 to + 28DepositorInsertGM-CSF promoter -627 to +28 fragment (CSF2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterGM-CSFAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-EGFP-CLASP2 340-1362
Plasmid#24372DepositorInsertCLASP2 (340-1362) (CLASP2 Human)
UseAdenoviralTagsEGFPExpressionMammalianMutationCLASP2 deletion mutant that retains MT lattice bi…PromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HSF1Δ∼PRD
Plasmid#71725Purposeplasmid expressing mic-his tag phosphorylation-deficient hHSF1DepositorInsertphosphorylation-deficient HSF1 (HSF1 Human)
UseTagsmac 6xHISExpressionMammalianMutation15 phosphorylation sites in RD mutated to AlaninePromoterAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2(AA)-NLS
Plasmid#192443PurposeEncodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.DepositorInsertmCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …PromoterCMVAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI
Plasmid#84257PurposeExpresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulationDepositorInsertsGAI-tagBFP-Sp dCas9-ABI
GID1-VPR
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …ExpressionMammalianMutationPromoterCAG and PGKAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV asyn S87A
Plasmid#36056DepositorInsertalpha-synuclein S87A (SNCA Human)
UseAAVTagsExpressionMammalianMutationS87APromoterCMVAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLPCX-Cx43-FS-IRES-GFP
Plasmid#65438PurposeMammalian expression of frameshift mutated Cx43 and EGFP separately driven by the ECMV IRES. For transfection or retroviral productionDepositorInsertCx43 (GJA1 Human)
UseRetroviralTagsIRES-GFPExpressionMammalianMutationframeshift (FS) mutation 35delAPromoterCMVAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only
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