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Plasmid#176341PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertSNAPC5 (SNAPC5 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTHAP12-N1.1.2-gDNA
Plasmid#175860PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertTHAP12-Nickase2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCHCHD3-N2.1.2-gDNA
Plasmid#175858PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertCHCHD3-Nickase2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCHCHD3-N1.1.1-gDNA
Plasmid#175857PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertCHCHD3-Nickase1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZNF449-donor
Plasmid#132515PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZNF449 (ZNF449 Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZNF668.1.0-gDNA
Plasmid#132463PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertZNF668 (ZNF668 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCENPBD1.1.0-gDNA
Plasmid#132460PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertCENPBD1 (CENPBD1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTHAP7.1.0-gDNA
Plasmid#132439PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertTHAP7 (THAP7 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalExpressionMutationPromoterTgTUB1 and Toxoplasma U6Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-pgaC
Plasmid#154919PurposeaTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymesDepositorInsertgRNA targeting pgaC
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpTetAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYZ673
Plasmid#132953PurposecrRNA entry vector of Pfba1-BsaI pad-TEF1 Term with FnCas12a in Schizosaccharomyces pombeDepositorInsertFnCas12a
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoteradh1Available sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYZ221
Plasmid#132952PurposecrRNA entry vector of Prrk1-Not1-HHR with FnCas12a in Schizosaccharomyces pombeDepositorInsertFnCas12a
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoteradh1Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-pheT
Plasmid#154920PurposeaTc inducible gRNA targeting pheT along with arabinose-inducible lambda red enzymesDepositorInsertgRNA targeting pheT
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-mqo
Plasmid#154921PurposeaTc inducible gRNA targeting mqo along with arabinose-inducible lambda red enzymesDepositorInsertgRNA targeting mqo
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-hisF
Plasmid#154922PurposeaTc inducible gRNA targeting hisF along with arabinose-inducible lambda red enzymesDepositorInsertgRNA targeting hisF
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-ampG
Plasmid#154923PurposeaTc inducible gRNA targeting ampG along with arabinose-inducible lambda red enzymesDepositorInsertgRNA targeting ampG
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC119-MCS
Plasmid#58807Purposecontains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSOX11-donor
Plasmid#176360PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertSOX11 (SOX11 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF219_1
Plasmid#72368PurposeEncodes gRNA for 3' target of human ZNF219 along with Cas9 with 2A GFPDepositorInsertZNF219 (ZNF219 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF219_2
Plasmid#72369PurposeEncodes gRNA for 3' target of human ZNF219 along with Cas9 with 2A GFPDepositorInsertZNF219 (ZNF219 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only