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Plasmid#67107PurposeStandard for cell-free expression benchmarking.DepositorInsertEHD2 (EHD2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationS320T, E384D, T387A, H388Q, T405V, V407A, H418R, …PromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCELF PAI-RBP v2
Plasmid#45505DepositorInsertPAI-RBP v2 (SERBP1 Human, Rat)
UseTagsGST tagExpressionMammalianMutationPromoterAvailable sinceJune 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
FRP792_insul-(lexA-box)2-PminCYC1-Citrine-TCYC1
Plasmid#58433PurposeInduces Citrine expression from a promoter containing 2 lexA boxes in yeast cellsDepositorInsertCitrineA206K
UseTagsExpressionYeastMutationPromoterinsul-(lexA-box)2-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
Mouse Alpha2 delta-2 mutMIDAS HA pMT2
Plasmid#58733Purposeexpression of HA tagged mouse Alpha2 delta-2 with MIDAS site mutations in mammalian cellsDepositorInsertalpha2 delta-2 (Cacna2d2 Mouse)
UseTagsExpressionMammalianMutationMIDAS site mutations (D300VSGS to AVAGA)PromoterSV40Available sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-mScarlet-SARS-CoV-2-5'UTR
Plasmid#184638PurposeFluorescent reporter for mammalian cells: Expression of mScarlet with SARS-CoV-2 5'UTRDepositorInsertmScarlet
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
TL1 alphaDdCBE 1-2 (right, DddA11-N)
Plasmid#222000PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-1/2 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233A | TALE: S1330I, A1341V,…PromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV14-3X-Flag-SARS-CoV-2 S
Plasmid#145780PurposeOver expression of SARS-CoV-2 S (spike) in mammalian cell lineDepositorInsertSARS-CoV-2 S (S SARS CoV 2)
UseTags3xFlagExpressionMammalianMutationCodon optimized and C-terminal 19 amino acids del…PromoterCMVAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a 6xHis PreScission SARS-CoV-2 nsp13
Plasmid#159390PurposeExpresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.DepositorInsertN-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
UseTagsHis6 tagExpressionBacterialMutationPromoterT7Available sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SARS-CoV-2-S-RBD-8his
Plasmid#145145PurposeMammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tagDepositorInsertSecreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
UseTags8his tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS SARS-CoV-2 BA.2.12.1 Spike
Plasmid#186032PurposeExpresses codon-optimized full length SARS-CoV-2 BA.2.12.1 SpikeDepositorInsertSARS-CoV-2 BA.2.12.1 Spike (S )
UseTagsExpressionMammalianMutationContains the BA.2 mutations (T19I, LPPA24S, G142D…Promoterchicken β-actin promoter, CMV enhancerAvailable sinceJune 8, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-KS-attB2-SA(2)-T2A-p65AD-Hsp70
Plasmid#62915PurposeCreating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2DepositorInsertT2A-p65AD-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)
Plasmid#62956PurposeSinglet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.DepositorInsertT2A-Gal4-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(2)-T2A-Gal4-Hsp70
Plasmid#62898PurposeCreating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2.DepositorInsertT2A-Gal4-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(2)-T2A-Gal4DBD-Hsp70
Plasmid#62904PurposeCreating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2DepositorInsertT2A-Gal4DBD-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(2)-T2A-dVP16AD-Hsp70
Plasmid#62911PurposeCreating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2DepositorInsertT2A-dVP16AD-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(2)-T2A-QF2-Hsp70
Plasmid#62946PurposeCreating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 2DepositorInsertT2A-QF2-Hsp70
UseTagsExpressionMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-2-GST-PD-1icd (AA194-288)
Plasmid#180814PurposeExpression of GST fused human PD-1 intracellular domain (AA194-288)DepositorInsertPD-1int (AA194-288) (PDCD1 Human)
UseTagsExpressionBacterialMutationPromoterTacAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pc-FLAGCSK-SH3-SH2 (2 to 190 amino acids)
Plasmid#74505PurposeOverexpression of CSK SH3-SH2 in mammalian cellsDepositorInsertCSK (CSK Human)
UseTagsFLAGExpressionMammalianMutationSH3-SH2 domainsPromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-noSV40-humanEC1.45_p300peak1-2_4XCoordinatorMutant#1-2-3-4
Plasmid#173992PurposeFirefly luciferase enhancer reporter plasmid.DepositorInsertp300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motifs #1, #2, #3 and #4 mutated
UseLuciferaseTagsExpressionMutation4X Coordinator motif mutations #1-2-3-4PromoterSV40 promoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS SARS-CoV-2 BA.4/5 Spike
Plasmid#186031PurposeExpresses codon-optimized full length SARS-CoV-2 BA.4/5 SpikeDepositorInsertSARS-CoV-2 BA.4/5 Spike (S )
UseTagsExpressionMammalianMutationBA.2 mutations (T19I, LPPA24S, G142D, V213G, G339…Promoterchicken β-actin promoter, CMV enhancerAvailable sinceJune 8, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits