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Intron-Tagging-pX330-Cas9-Blast

Plasmid

#159741

Purpose

Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

Depositor

Stefan Kubicek

Article

Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

Insert

donor plasmid targeting sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-VP64-dCas9-VP64_Blast

Plasmid

#192650

Purpose

3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

VP64-dCas9-VP64

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1a

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGMC00025 (aka pCW-dCas9-Blast)

Plasmid

#195316

Purpose

Dox inducible lentiviral dead Cas9

Depositor

Raj Chari

Insert

dead Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

D10A; H840A

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLL8 lenti-D10Acas9-3xflag-blast

Plasmid

#112134

Purpose

lenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

D10Acas9-3xflag-blast

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A mutant in Cas9

Promoter

EF1a

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti CMVtight Blast DEST (w762-1)

Plasmid

#26434

Depositor

Eric Campeau

Type

Empty backbone

Use

Lentiviral; Tet-on advanced destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2011

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-HNHx-ABE8eHD-BlastR

Plasmid

#176478

Purpose

A-to-G base editor with alternative targeting scope

Depositor

Gerald Schwank

Article

Villiger et al Mol Ther Nucleic Acids. 2021 Aug 26;26:5

Insert

HNHx-ABE8eHD

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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BcLOV4 fusion tools screening plasmid #1

Plasmid

#174511

Purpose

Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP in a pcDNA3.1 backbone.

Depositor

Brian Chow

Article

Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

Insert

[BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a(+)_beta2M (MHC Class I light)

Plasmid

#187357

Purpose

Bacterial expression and refolding of gp100 pMHC

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

beta2M (MHC Class I light)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGHUC w-ERBIN (2-hybrid prey plasmid)

Plasmid

#128163

Purpose

UV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).

Depositor

Marcus Noyes

Article

Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

Insert

ERBIN PDZ domain (ERBIN Synthetic)

Use

Synthetic Biology

Tags

FLAG and Omega subunit of RNA polymerase

Expression

Bacterial

Mutation

Promoter

UV5

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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BcLOV4 fusion tools screening plasmid #2

Plasmid

#174512

Purpose

Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.

Depositor

Brian Chow

Article

Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

Insert

plasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP

Use

Tags

3xFLAG and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-ACE2-his expression plasmid

Plasmid

#158089

Purpose

Expresses ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

ACE2 (ACE2 Human)

Use

Tags

Expression

Mammalian

Mutation

See depositor comments below

Promoter

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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lentiTet-OTX2-P2A-mCherry BlastR

Plasmid

#186741

Purpose

Dox inducible OTX2-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Insert

OTX2 (OTX2 Human)

Use

Lentiviral

Tags

P2A-mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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p2T-cmv-SpCas9-TadCBEd-BlastR

Plasmid

#193848

Purpose

Express TadCBEd in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEd

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRC10-EFS-huLbCpf1-2A-Blast-WPRE

Plasmid

#123359

Purpose

Lentiviral vector to constitutively express humanized LbCpf1/Cas12a, with blasticidin resistance for selection.

Depositor

Sidi Chen

Article

Chow et al Nat Methods. 2019 May;16(5):405-408. doi

Insert

human codon-optimized Cpf1/Cas12a from Lachnospiraceae bacterium,

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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Dual opposing T7 promoters reporter plasmid

Plasmid

#156457

Purpose

A mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Insert

NeoR/KanR

Use

Tags

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

Em7

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-ADAM22 (cytoplasmic) [N46/30R]

Plasmid

#177555

Purpose

Mammalian Expression Plasmid of anti-ADAM22 (cytoplasmic) (Mouse). Derived from hybridoma N46/30.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-ADAM22 (cytoplasmic) (Mus musculus) recombinant mouse monoclonal antibody (Adam22 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti CMVtight eGFP Blast (w782-1)

Plasmid

#26584

Purpose

2nd generation lentiviral vector

Depositor

Eric Campeau

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral; Tet-on advanced vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2011

Availability

Academic Institutions and Nonprofits only

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p2T-cmv-SpCas9-TadDE-BlastR

Plasmid

#193850

Purpose

Express TadDE in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadDE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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p2T-cmv-SpCas9-TadCBEa-BlastR

Plasmid

#193847

Purpose

Express TadCBEa in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEa

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-RhoB Sensor-IRES-Blast

Plasmid

#222633

Purpose

Rho reporter

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

RhoB Sensor

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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