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Addgene
Showing: 821 - 840 of 1875 results

  1. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)

    Plasmid
    #169077
    Purpose
    Insect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepII
    Depositor
    Sascha Martens
    Article
    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151
    Inserts
    ATG5
    ATG7
    ATG10 (ATG10 Human)
    ATG12
    ATG16 (ATG16L1 Human)
    Use
    Tags
    GFP tag, GGlinker, TEV cleavage site. StrepII and…
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Polyhedrin promoter
    Availability
    Academic Institutions and Nonprofits only
    169077-plasmid-map-sequence-id-332050

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  2. hTLR4

    Plasmid
    #13086
    Depositor
    Ruslan Medzhitov
    Insert
    TLR4 (TLR4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    13086-plasmid-map-sequence-id-142442

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  3. pME-dCas9-BFP-KRAB

    Plasmid
    #221502
    Purpose
    CRISPRi machinery fish codon optimized
    Depositor
    Alessandro Ori
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Tags
    Expression
    Mutation
    D10A and H839A point mutations
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    221502-plasmid-map-sequence-id-439413

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  4. pcDNA3-TLR4-YFP

    Plasmid
    #13018
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 4 (TLR4 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    13018-plasmid-map-sequence-id-339915

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  5. pET11a-p52(1-398)

    Plasmid
    #44733
    Depositor
    Gourisankar Ghosh
    Insert
    p52 (NFKB2 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    AA 1-398
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only

  6. pHis8-p100(1-899)

    Plasmid
    #44735
    Depositor
    Gourisankar Ghosh
    Insert
    p100 (NFKB2 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    AA 1-899
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  7. pCLXSN(GFP)-hp100

    Plasmid
    #174734
    Purpose
    Gene overexpression
    Depositor
    Shao-Cong Sun
    Insert
    P100 (NFKB2 Human)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    174734-plasmid-map-sequence-id-343213

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  9. pcDNA3-His-SUMO3

    Plasmid
    #159325
    Purpose
    Mammalian cell expression of mouse His-SUMO3
    Depositor
    Jianjun Zhao
    Insert
    His-SUMO3 (Sumo3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    159325-plasmid-map-sequence-id-311868

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  10. pPD1101 Integration Test Circuit Vector

    Plasmid
    #139251
    Purpose
    Integration vector with TUs incorporated. Cells will be red and, if treated with doxycycline, blue.
    Depositor
    Joshua Leonard
    Insert
    Tus
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    139251-plasmid-map-sequence-id-337940

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  11. pITI1

    Plasmid
    #166094
    Purpose
    Gal inducible expression of dCas9-FokI
    Depositor
    Rebecca Burgess
    Insert
    dCas9-FokI
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    spCas9-D10A, H840A
    Promoter
    Gal-inducible
    Availability
    Academic Institutions and Nonprofits only
    166094-plasmid-map-sequence-id-329610

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  12. pRR2

    Plasmid
    #166097
    Purpose
    Gal inducible expression of dCas9-FokI
    Depositor
    Rebecca Burgess
    Insert
    dCas9-FokI
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    spCas9-D10A, H840A
    Promoter
    Gal-inducible
    Availability
    Academic Institutions and Nonprofits only
    166097-plasmid-map-sequence-id-329109

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  13. pCfb13220

    Plasmid
    #219895
    Purpose
    The base plasmid of TUNEYALI for TF30
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF30 (YALI1_D10007g) and homologous arm matching TF30
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    219895-plasmid-map-sequence-id-437825

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  14. NM-01

    Plasmid
    #69799
    Purpose
    Bacterial expression of Cas9 with His, MBP, and Strep tags
    Depositor
    Gerard Cagney
    Insert
    Cas9
    Use
    Tags
    HIS, MBP, and StrepII
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine (D10A) and Histidine 840 …
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  15. pLenti-ABE7.10-P2A-Puro

    Plasmid
    #112674
    Purpose
    Lentivirus for constitutive expression of ABE7.10 (not codon optimized)
    Depositor
    Lukas Dow
    Insert
    ABE7.10
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    D10A
    Promoter
    EF1s
    Availability
    Academic Institutions and Nonprofits only
    112674-plasmid-map-sequence-id-215665

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  16. Lenti-EF1alpha-dCas9-KRAB_Hygro

    Plasmid
    #192663
    Purpose
    3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance marker
    Depositor
    Jeffrey Miner
    Insert
    dCas9-KRAB
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, D839A, H840A and N863A in Cas9
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    192663-plasmid-map-sequence-id-379915

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  17. pTRE3G-VP64-dCas9-VP64-ZsG

    Plasmid
    #192668
    Purpose
    Dox-inducible expression of VP64-dCas9-VP64 with 2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    VP64-dCas9-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    TRE3G
    Availability
    Academic Institutions and Nonprofits only
    192668-plasmid-map-sequence-id-379892

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  18. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid
    #195316
    Purpose
    Dox inducible lentiviral dead Cas9
    Depositor
    Raj Chari
    Insert
    dead Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    D10A; H840A
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    195316-plasmid-map-sequence-id-385750

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  19. WT_S1-H1

    Plasmid
    #218392
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Availability
    Academic Institutions and Nonprofits only
    218392-plasmid-map-sequence-id-436407

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  20. WT_S7-H1

    Plasmid
    #218393
    Purpose
    3-exon minigene, with SRSF7 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Availability
    Academic Institutions and Nonprofits only
    218393-plasmid-map-sequence-id-433906

    Enlarge

Showing: 821 - 840 of 1875 results