Addgene

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pcA12/F527

Plasmid

#17683

Depositor

David Shalloway

Article

Yaciuk et al Mol Cell Biol. 1989 Jun . 9(6):2453-63.

Insert

c-src S12A Y527F (SRC Chicken)

Use

Tags

Expression

Mammalian

Mutation

Ser 12 changed to Ala. Tyr 527 changed to Phe .

Promoter

Available since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
ZP47 Nlk1

Plasmid

#16895

Depositor

Randall Moon

Article

Thorpe et al Development. 2004 Jun;131(12):2899-909.

Insert

Nlk1 (nlk1 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
p218 pCMV-CREM-L

Plasmid

#8396

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CREM-L

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

modified Cre, single LOX 511 site

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pSKB3.MPN314

Plasmid

#11433

Depositor

Sung-Hou Kim

Article

Chen et al Proteins. 2004 Jun 1. 55(4):785-91.

Insert

cell division protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pWUKI Cas1 Cas2 E9Q

Plasmid

#80091

Purpose

Encodes Cas1 and Cas2 E9Q

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array (bp1542-2899)

Use

Tags

Expression

Bacterial

Mutation

E9Q

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pWUKI Cas1 V134L Cas2

Plasmid

#80092

Purpose

Encodes Cas1 V134L and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Use

Tags

Expression

Bacterial

Mutation

V134L

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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pSL7 - Brr2 del247 N1104L

Plasmid

#90257

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Use

Tags

TAP

Expression

Yeast

Mutation

deleted first 247 amino acids and N1104L

Promoter

Available since

Availability

Academic Institutions and Nonprofits only
pLJM1-EGFP

Plasmid

#19319

Purpose

3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pLKO.1 GFP shRNA

Plasmid

#30323

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

GFP shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR CD22-Megf10 CAR

Plasmid

#113016

Purpose

for production of lentivirus encoding CD22 CAR-P Megf10

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD22 CAR-P Megf10 intracellular domain

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Cra-GFP

Plasmid

#220151

Purpose

To track the natural Cra promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

Cra promoter only

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CRP-GFP

Plasmid

#220152

Purpose

To track the natural CRP promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

CRP promoter only

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Fis-GFP

Plasmid

#220153

Purpose

To track the natural Fis promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

Fis promoter only

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Lrp-GFP

Plasmid

#220158

Purpose

To track the natural Lrp promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

Lrp promoter only

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MarA-GFP

Plasmid

#220159

Purpose

To track the natural MarA promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

MarA promoter only

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NsrR-GFP

Plasmid

#220162

Purpose

To track the natural NsrR promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

NsrR promoter only

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SoxS-GFP

Plasmid

#220165

Purpose

To track the natural SoxS promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

SoxS promoter only

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LexA-GFP

Plasmid

#220157

Purpose

To track the natural LexA promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

LexA promoter only

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Fur-GFP

Plasmid

#220156

Purpose

To track the natural Fur promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

Fur promoter only

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PhoB-GFP

Plasmid

#220163

Purpose

To track the natural PhoB promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

PhoB promoter only

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FlhD-GFP

Plasmid

#220154

Purpose

To track the natural FlhD promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

FlhD promoter only

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Fnr-GFP

Plasmid

#220155

Purpose

To track the natural Fnr promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

Fnr promoter only

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PhoP-GFP

Plasmid

#220164

Purpose

To track the natural PhoP promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

PhoP promoter only

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NarL-GFP

Plasmid

#220160

Purpose

To track the natural NarL promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

NarL promoter only

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC18R6KT-mini-Tn7T-Km

Plasmid

#64969

Purpose

mini-Tn7 base vector with transcriptional terminator and oriT

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBABE-puro

Plasmid

#1764

Purpose

Retroviral vector for cloning and expressing your gene of interest. Puromycin selection.

Depositor

Hartmut Land, Jay Morgenstern, Bob Weinberg

Article

Morgenstern et al Nucleic Acids Res. 1990 Jun 25;18(12):35

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
TRE-ChrimsonR-mCherry

Plasmid

#92207

Purpose

Reporter construct

Depositor

Alice Ting

Article

Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

ChimsonR-mCherry

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-EF1alpha-dCas9-p300_Blast

Plasmid

#192653

Purpose

3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

dCas9-p300

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A; H840A in Cas9

Promoter

EF1a

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pME18S-F-R-Olfr160

Plasmid

#149366

Purpose

Expresses Olfr160 in mammalian cells.

Depositor

Takeshi Imai

Article

Inagaki et al Cell Rep. 2020 Jun 30;31(13):107814. doi

Insert

Olfr160 (Olfr160 Mouse)

Use

Tags

FLAG and rhodopsin

Expression

Mammalian

Mutation

Promoter

SR alpha

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330A-1x2

Plasmid

#58766

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pKIKOarsBKm

Plasmid

#46766

Purpose

E.coli genomic integration at arsB

Depositor

Claudia Vickers, Lars Nielsen

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Tags

Expression

Mutation

Promoter

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pG12-SEAP

Plasmid

#170271

Purpose

Secreted alkaline phosphatase (SEAP) expression in mammalian cells. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

SEAP

Use

Tags

Expression

Mammalian

Mutation

Promoter

12xUAS with TATA-box minimal promoter

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABe-HA-ER-IPpoI

Plasmid

#32565

Depositor

Michael Kastan

Article

Berkovich et al Nat Cell Biol. 2007 Jun;9(6):683-90. Epu

Insert

I-PpoI

Use

Retroviral

Tags

ER and HA

Expression

Mammalian

Mutation

none

Promoter

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pKIKOrbsARCm

Plasmid

#46765

Purpose

E.coli genomic integration rbsAR locus

Depositor

Claudia Vickers, Lars Nielsen

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pKIKOarsBCm

Plasmid

#46763

Purpose

E.coli genomic integration at arsB

Depositor

Claudia Vickers, Lars Nielsen

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330S-2

Plasmid

#58778

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pKIKOrbsARKm

Plasmid

#46768

Purpose

E.coli genomic integration rbsAR locus

Depositor

Claudia Vickers, Lars Nielsen

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
EasyFusion T2A-H2B-miRFP703

Plasmid

#113097

Purpose

General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-miRFP703

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-hygro

Plasmid

#1765

Purpose

Retroviral vector for cloning and expressing your gene of interest. Hygromycin selection.

Depositor

Hartmut Land, Jay Morgenstern, Bob Weinberg

Article

Morgenstern et al Nucleic Acids Res. 1990 Jun 25;18(12):35

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ463

Plasmid

#187971

Purpose

TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

Depositor

Jef Boeke

Article

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIG-PP2A-Abeta

Plasmid

#15248

Depositor

William Hahn

Article

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

PP2A regulatory subunit A, beta isoform (PPP2R1B Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pCAG-Lyn11-FRB

Plasmid

#155228

Purpose

membrane anchor for FKBP-FRB system

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Membrane targeted (through Lyn11) FRB for use with FKBP-FRB dimerization system

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-mNeon-TurboID

Plasmid

#186610

Purpose

Third generation lentiviral vector expressing mNeonGreen fused to V5-TurboID

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

mNeon-V5-TurboID

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

For reference see Branon et al., 2018

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
psfaMs-Tn5

Plasmid

#183637

Purpose

Expresses anti-mouse nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Article

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti mouse secondary nanobody fused to Tn5

Use

Tags

anti-mouse nanobody

Expression

Bacterial

Mutation

Promoter

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAD01 S. cerevisiae telomerase RNA KBS

Plasmid

#61890

Purpose

Plasmid for run-off in vitro transcription of Saccharomyces cerevisiae KBS RNA

Depositor

Thomas Cech

Article

Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

Insert

Telomerase RNA hairpin

Use

Tags

Expression

Mutation

Promoter

T7

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pKm-HABE

Plasmid

#170332

Purpose

a modularized single-plasmid adenosine base editing (A-to-G) tool for Sinorhizobium meliloti

Depositor

Deqiang Duanmu

Article

Wang et al Front Microbiol. 2021 Jun 18;12:686008.

Insert

Rhizobium codon-optimized ABEs

Use

CRISPR

Tags

Expression

Mutation

Cas9n-D10A

Promoter

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
T444T

Plasmid

#113081

Purpose

Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.

Depositor

Tibor Vellai

Article

Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

Type

Empty backbone

Use

RNAi

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PCS2+Cas9-mSA

Plasmid

#103882

Purpose

Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

CAS9-MSA

Use

CRISPR

Tags

MSA (monomeric streptavidin

Expression

Mutation

none

Promoter

SP6

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHPyV7-713a

Plasmid

#24728

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

Full genome of Human polyomavirus 7

Use

Viral clone

Tags

Expression

Mutation

Promoter

Available since

June 8, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pHPyV6-607a

Plasmid

#24727

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

Full genome of Human polyomavirus 6

Use

Viral clone

Tags

Expression

Mutation

Promoter

Available since

June 8, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge

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