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pSLIK-PUM1-rABE-Flag

Plasmid

#191385

Purpose

Doxycycline inducible expression of PUM1-rABE

Depositor

Stephen Floor

Article

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

PUM1-rABE (PUM1 Synthetic, Human)

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

tet-inducible promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-Puro-hTBK1-EGFP

Plasmid

#221553

Purpose

Expresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

Insert

TBK1 (TBK1 Human)

Use

Piggybac

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pEn-Chimera

Plasmid

#61432

Purpose

Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

AtU6-26:sgRNA

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU6-26

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-dCas9-T2A-GFP

Plasmid

#193781

Purpose

Tricistronic expression of dCas9-EGFP-BSD

Depositor

James Eshleman

Article

Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

Insert

dead Cas9

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

In frame with existing Cas9 promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAJB6a

Plasmid

#19502

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

DNAJB6a (DNAJB6 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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opto-Cdc42

Plasmid

#174510

Purpose

Expresses opto-Cdc42 (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 backbone.

Depositor

Brian Chow

Article

Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

Insert

opto-Cdc42-mCherry

Use

Tags

3xFLAG and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pT040

Plasmid

#67640

Purpose

URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette

Depositor

John Wyrick

Article

Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

Insert

single guide RNA expression cassette

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

pSNR52

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ141B2.0

Plasmid

#99897

Purpose

Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU3

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIP-BSR-GFP11

Plasmid

#68716

Purpose

Split GFP assay

Depositor

Yutaka Hata

Article

Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

Insert

bsr

Use

Retroviral

Tags

FLAG and GFP11

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pDestattB (pCM268)

Plasmid

#68313

Purpose

Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish

Depositor

Leonard Zon, Christian Mosimann

Article

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Type

Empty backbone

Use

Zebrafish transgenesis

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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