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Plasmid#199459PurposeU6-driven crRNA targeting TS1 sequence (CCTCCTCGGAGAGCATCGGTGC )DepositorInsertTS1 crRNA
UseCRISPRTagsExpressionMutationPromotermouse U6Available sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA17-CRISPR-Tag (11XTS1) for dSpCas9
Plasmid#199448PurposeCRISPR-Tag sequence that can be efficiently bound by dSpCas9DepositorInsertCRISPR-Tag
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
SP_H6_Halo_L172Q_KDEL_pBABEpu
Plasmid#183690PurposeRetroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloL172Q)DepositorInsertER Halo(L172Q)
UseRetroviralTagsExpressionMutationL72Q in HalotagPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
SP_H6_Halo_M21K_F86L_KDEL_pBABEpu
Plasmid#183691PurposeRetroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloM21K_F86L)DepositorInsertER Halo(M21K_F86L)
UseRetroviralTagsExpressionMutationM21K_F86L in HalotagPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30_Halotag_K73T_Jdomain
Plasmid#183693PurposeBacterial expression of ER-targeted metastable Halotag/folding sensor (HaloK73T) fused with J domainDepositorInsertER Halo(K73T)-J domain
UseTagsExpressionBacterialMutationK73T in HalotagPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-αC
Plasmid#197650PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-2 αC
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationSer677T substitutionPromoterADH1Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-HsSmg6-mut-siRNAres_I
Plasmid#146588PurposeMammalian Expression of HsSmg6-mut-siRNAresDepositorInsertHsSmg6-mut-siRNAres (SMG6 Human)
UseTagsExpressionMammalianMutationtwo silent mutations T594C, A699G and two non sil…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORE303N_CEN12Fuzzy3MYB
Plasmid#194440PurposeCRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12 and Fuzzy3MYBDepositorInsertgRNA array targeting CEN1, Fuzzy3MYB
UseCRISPRTagsExpressionPlantMutationPromoterMtU6Available sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
HySp5-310
Plasmid#192995PurposePlasmid that encodes for Hydra Sp5, lacking the SP boxDepositorInsertHydra Sp5
UseTagsHAExpressionMammalianMutationno SP boxPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
VPS13C^halo∆1235-1748
Plasmid#187297PurposeExpress VPS13C truncation mutant in mammalian cellsDepositorInsertVPS13C truncation mutant (VPS13C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORE303N_CEN1
Plasmid#194439PurposeCRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout CEN1DepositorInsertgRNA targeting CEN1
UseCRISPRTagsExpressionPlantMutationPromoterMtU6Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1093B
Plasmid#194003PurposepBac-U6-crCHIKV-array-UTR-3xp3-tdTomatoDepositorInsert4 gRNAs targeting CHIKV
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
OA-1085F
Plasmid#191375PurposepBac-U6-crYellow-array-UTR-3xp3-tdTomatoDepositorInsert4 gRNAs targeting yellow
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC2
Plasmid#191857PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003_sgTFAP4 guide 2
Plasmid#193587PurposeTFAP4 knockoutDepositorInsertsgTFAP4 guide 2 (TFAP4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC3
Plasmid#191858PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T-Easy-HySp5-557
Plasmid#193000PurposePlasmid to generate an ISH probe for Hydra Sp5DepositorInsertHydra Sp5
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZfSp5a-377
Plasmid#192997PurposePlasmid that encodes for Zebrafish Sp5DepositorInsertZebrafish Sp5
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
HySp5-227
Plasmid#192996PurposePlasmid that encodes for Hydra Sp5, lacking the SP box and DNA binding domainDepositorInsertHydra Sp5
UseTagsHAExpressionMammalianMutationno SP box and DNA binding domainPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
HySp5:Luc
Plasmid#192985PurposePlasmid to test the Hydra Sp5 promoter activityDepositorInsertHydra Sp5 promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only