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Plasmid#100091PurposedCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expressionDepositorTypeEmpty backboneUseCRISPRTags3XFlag-NLS-dCas9-NLSExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only
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TFORF2772
Plasmid#144233PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKDM5B (KDM5B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-IL-2 signal
Plasmid#128028PurposeMammalian expression vector with N terminal IL-2 signal peptideDepositorTypeEmpty backboneUseTagsIL-2 signal peptideExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B6/7 ∆E3-GFP
Plasmid#179202PurposeA block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6∆E3-GFP and pAd5-B7.DepositorInsertModified block 6/7, with deletion in E3 and insertion of GFP cassette
UseAdenoviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-P2A-K0-P2A-RFP
Plasmid#105686PurposeDouble fluorescent stalling reporter K0DepositorInsertsP2A-3xFLAG-VHPbeta
K0
P2A-mCherry
UseTagsExpressionMammalianMutationPromoterCMV (from vector)Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_EWSR1_ERG
Plasmid#205819PurposeExpress mEGFP-tagged fusion protein, EWSR1_ERG from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB411
Plasmid#74805PurposeGateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
EGFP gRNA (BRDN0000563266)
Plasmid#80036Purpose3rd generation lentiviral gRNA plasmid targeting EGFPDepositorInsertEGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-APOBEC1-YTH
Plasmid#131636PurposeExpresses APOBEC1 fused to the YTH domainDepositorInsertAPOBEC1 fused to YTH domain (YTHDF2 Human, Rat)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE6e
Plasmid#207855PurposeMammalian expression of PE6e prime editorDepositorInsertPE6e
UseTagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationCas9(H840A, K918A, K775R)PromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only