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Plasmid#15574DepositorInserthtt25Q + Pro (HTT Human)
UseTagsCFPExpressionYeastMutationProline-rich region presentPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense
Plasmid#46839PurposeExpress cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translatedDepositorInsertCoral Green Fluorescent Protein (cGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-RAP1
Plasmid#84525PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationno stop codonPromoternoneAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJG486
Plasmid#91195PurposeWDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi1+donor)DepositorInsertnTaCas9_H840A+gUbi1+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
SMT205
Plasmid#134817PurposeSMT201 with mutated RBS for DHFR, in vivo assaysDepositorInsertsDihydrofolate reductase
Thymidylate synthase
UseTagsExpressionBacterialMutationSilent mutations to eliminate internal restrictio…PromoterT7 and TETAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAPi
Plasmid#127548PurposeEmpty control for pGAPi-based RNAi silencing in tandem with APT transcript segmentDepositorInsertAdenine Phosphorybosyltransferase transcript segment
UseRNAi; Empty controlTagsExpressionMutationPromoterUbiquitinAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
MyoUTipGAPi
Plasmid#127549PurposePositive RNAi control for pGAPi-based RNAi silencing in tandem with APT transcript segmentDepositorInsertMyosin XIa+b 5'UTR and Adenine Phosphorybosyltransferase transcript segment
UseRNAi; Positive controlTagsExpressionMutationPromoterUbiquitinAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTC393
Plasmid#91221Purposeprotoplast vector expressing gRNA24 targeting tomato ANT1 (with TREX2 expression)DepositorInsertgRNA targeting tomato ANT1
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC363
Plasmid#91211Purposeprotoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtU6 promoters)DepositorInsert2 gRNAs targeting tomato ARF8A
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-mIFITM3
Plasmid#58458PurposeExpresses murine IFITM3 with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMV IEAvailable sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0701
Plasmid#91019PurposeModule A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_H840A nickase
UseCRISPRTagsExpressionMutationH840APromoter35SAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
GAL HA25QDProCFPp303
Plasmid#15578DepositorInserthtt 25Q Delta Pro (HTT Human)
UseTagsCFP and HAExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
GAL HA103QDProCFPp303
Plasmid#15579DepositorInserthtt 103Q Delta Pro (HTT Human)
UseTagsCFP and HAExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-C105A
Plasmid#58392PurposeExpressed murine IFITM3-C105A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationCysteine 105 to AlaninePromoterCMV IEAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1405
Plasmid#53010PurposepLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
DsrA sRNA
hfq
sodB
UseSynthetic BiologyTagsgfpExpressionBacterialMutationcontains -56 to +141 relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
phNFL-N98S
Plasmid#132592PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E N98S mutationDepositorInsertNEFL (NEFL Human)
UseTagsExpressionMammalianMutation293A>G; Asn98SerPromoterCMVAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
824 pGEX2T PTEN delta274-342
Plasmid#10739DepositorInsertPTEN (PTEN Human)
UseTagsGSTExpressionBacterialMutationDeletion of amino acids 274-342.PromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
Cenp-B DBD INCENP TSS/AAA mCherry
Plasmid#45234PurposeTarget INCENP (with TSS mutated to AAA) to centromeres via CENP-B DNA binding domain (DBD)DepositorInsertCenp-B DBD, INCENP-ΔCEN with AAA mutation, mCherry
UseTagsCENP-B DBD, DNA binding domain of CENP-B and mChe…ExpressionMammalianMutationINCENP C-terminal TSS mutated to AAAPromotert7Available sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKP4010
Plasmid#113336PurposeBeta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoterDepositorInsertsArtificial Transcription Factor (McIsaac et al, 2013)
Ty3 Integrase
UseTags3X-FLAG/8X-Gly linkerExpressionYeastMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
px330-MRPsg4
Plasmid#87192Purposedisruption of human RMRP gene via CRISPR-Cas9, guide 4DepositorInserthuman MRP RNA CRISPR guide #4 (RMRP Human)
UseCRISPRTagsExpressionMammalianMutationPromoterhuman U6Available sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only