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Plasmid#171622PurposeTRE-ChR2-YFP reporterDepositorInsertTRE-ChR2-YFP
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterTetOAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hMLN
Plasmid#27079PurposeNon-integrating (episomal) expression of human C-MYC, LIN28 and NANOGDepositorInsertC-MYC, LIN28, NANOG (MYC Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA2-DDB1
Plasmid#19909DepositorInsertDamage-specific DNA binding protein 1 (DDB1 Human)
UseTagsHAExpressionMammalianMutationplease see depositor comment belowPromoterAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1a-c-Met
Plasmid#31784DepositorInsertMet (MET Human)
UseTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
(559) pAAV EF-1a KRAB-SadCas9 U6-gRNA
Plasmid#163024PurposeExpression of CRISPRi with U6-gRNA (Bsa1 sites)DepositorInsertMammalian codon-optimized SadCas9
UseAAVTagsKRAB, SV40 polyA, and myc NLSExpressionMammalianMutationPromoterhEF-1aAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
attB-mCh-Poly(A)
Plasmid#65525PurposePlasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-mCherry
UseMouse Targeting; Bxb1TagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pK168.AAV-EF1α-DIO-tTA-P2A-RFP-WPRE (Supernova)
Plasmid#85039PurposeFor AAV-TRE-Cre based Supernova-RFP, pK168 should be used with pK170.DepositorInsertRFP-P2A-tTA
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB-BRCA2
Plasmid#99395PurposeMammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA2DepositorInsertBRCA2 (BRCA2 Human)
UseTagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpeCas9-HF
Plasmid#192141PurposeExpresses highly specific SpeCas9, and cloning backbone for sgRNADepositorInsertSpeCas9-HF
UseAAVTagsExpressionMammalianMutationSpeCas9-HF (R247A, N415A, S421A)PromoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
PIK3CA - Exon20 H1047R
Plasmid#16639DepositorInsertPIK3CA H1047R (PIK3CA Human)
UseTagsFlagExpressionMammalianMutationH1047RPromoterAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGL3b-8XGliBS:EGFP
Plasmid#84602PurposeGli protein-dependent EGFP reporter for analysis of Hedgehog signalingDepositorInserteGFP
UseTagsExpressionMammalianMutationPromoter8X Gli-binding siteAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pyc126m
Plasmid#158777Purposeribosome-tagged GCaMP for soma localizationDepositorInsertGCaMP6m-RPL10a
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha1 P35S:PhiC31 integrase-RDF:T35S (GB2893)
Plasmid#160633PurposeTU for the constitutive expression of the PhiC31 integrase, C-terminally fused with its reversion factor (RDF)DepositorInsertPhiC31-RDF
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pN1-CMV-sDarken
Plasmid#184799PurposeSerotonin Sensor sDarken under the control of a CMV Promoter.DepositorInsertsDarken
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Gluc-RMA-IRES-EGFP
Plasmid#189629PurposeExpresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction.DepositorInsertsGaussia luciferase fused to Fc
IRES-EGFP
UseAAV and LuciferaseTagsGluc and IgG1 FcExpressionMammalianMutationPromoterIRES and hSynAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS1ClacZ
Plasmid#55171PurposelacZ-reporter vector, integration at amyE, ampr, cmrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoternoneAvailable sinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
cyto-YFP-FKBPx5
Plasmid#103777Purposeexpression of iPOLYMER component in mammalian cells; has nuclear exclusion sequenceDepositorInsertcyto-YFP-FKBPx5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-cofilin WT
Plasmid#78295PurposeMammalian expression of mouse cofilin fused to EGFPDepositorInsertcofilin 1 (Cfl1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET41sIII_GST-ALC1
Plasmid#173932PurposeBacterial expression of ALC1DepositorInsertALC1 (CHD1L Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDonor-tBFP-NLS-Neo (Universal)
Plasmid#80767PurposeUniversal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.DepositorInsertPITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only