Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 8321 - 8340 of 16034 results

  2. FB027

    Plasmid
    #119712
    Purpose
    Multipartite assembly obtained by combining FB parts FB026+FB003 into pDGB3omega1. Used for fluorescent tagging of fungi (Hyg resistance +YFP) according to FungalBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    PgpdA::YFP::TtrpC(←)-PtrpC::hph::Ttub (→)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    119712-plasmid-map-sequence-id-236968

    Enlarge

  3. pGEM-NLS-Cre-2A-mCherry-FKF

    Plasmid
    #119868
    Purpose
    Donor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Use
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    119868-plasmid-map-sequence-id-240979

    Enlarge

  8. EGFP-acVHH (Y34F)-PB

    Plasmid
    #171071
    Purpose
    A COSMO mutant (Y34F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody variant
    Use
    Tags
    modified STIM1–PB tag
    Expression
    Mammalian
    Mutation
    Y34F
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    171071-plasmid-map-sequence-id-338141

    Enlarge

  9. EGFP-acVHH (Y34W)-PB

    Plasmid
    #171072
    Purpose
    A COSMO mutant (Y34W) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody variant
    Use
    Tags
    modified STIM1–PB tag
    Expression
    Mammalian
    Mutation
    Y34W
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    171072-plasmid-map-sequence-id-338143

    Enlarge

  14. pEGFP-C1-IFT57

    Plasmid
    #218728
    Purpose
    Expresses N-terminally EGFP-tagged IFT57 in mammalian cells
    Depositor
    Kazuhisa Nakayama
    Insert
    IFT57 (IFT57 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    218728-plasmid-map-sequence-id-440876

    Enlarge

  17. GABA(A) receptor subunit g2SE

    Plasmid
    #49170
    Purpose
    pHluorin-tagged GABA A receptor subunit (gamma 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Tija Jacob, Stephen Moss
    Article
    Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.
    Insert
    GABA(A) receptor subunit gamma 2 (Gabrg2 Mouse)
    Use
    Tags
    myc, pHGFP (pHluorin), and thrombin cleavage site…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49170-plasmid-map-sequence-id-165320

    Enlarge

  18. GABA(A) receptor subunit B3SE

    Plasmid
    #49171
    Purpose
    pHluorin-tagged GABA A receptor subunit (beta 3) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Tija Jacob, Stephen Moss
    Article
    Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.
    Insert
    GABA(A) receptor subunit beta 3 (Gabrb3 Mouse)
    Use
    Tags
    myc, pHGFP (pHluorin), and thrombin cleavage site…
    Expression
    Mammalian
    Mutation
    GTG (V) to TGC (C)
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    49171-plasmid-map-sequence-id-72502

    Enlarge

Showing: 8321 - 8340 of 16034 results