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Plasmid#171621PurposeTRE-NpHR-EYFP reporterDepositorInsertTRE-NpHR-EYFP
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterTetOAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR_DKO
Plasmid#183192PurposePlasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screenDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6, mU6Available sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-GBF1
Plasmid#206415PurposeMammalian expression of hGBF1DepositorInsertGBF1 (GBF1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBMN HA-NDP52
Plasmid#199307PurposeFor expression of the mammalian NDP52 tagged with HADepositorInsertNDP52 (CALCOCO2 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP
Plasmid#158962PurposeCre-dependet Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed DsRed cassette
Cas9-GFP
UseCRISPR and Cre/LoxTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-DIO-PSD95.FingR-eGFP-CCR5TC
Plasmid#126216PurposeLabeling of excitatory synapses (green fluorescence) in cre-expressing neuronsDepositorInsertPSD95.FingR-eGFP-CCR5TC
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1-(151-241)
Plasmid#24393DepositorInsertBeclin1 (BECN1 Human)
UseTagsFLAGExpressionMammalianMutationAmino acids 151-241 are deleted. Delta CC mutant.PromoterAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher
Plasmid#117656PurposeBacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.DepositorInsertssfCherry2(1-10)
sfCherry2(11)-SpyCatcher002
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
peGFP C3-SPG20-PAAA
Plasmid#218581PurposeExpresses GFP-tagged PPAY motif mutant (PAAA) of human SPG20 in mammalian cells; this mutant does not interact with ITCHDepositorInsertSPG20 (SPART Human)
UseTagseGFPExpressionMammalianMutationchanged Proline 172 to Alanine and Tyrosine 174 t…PromoterCMVAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
peGFP C1-SPG20 SC domain
Plasmid#218580PurposeExpresses GFP-tagged SC domain of human SPG20 in mammalian cellsDepositorInsertSPG20 (SPART Human)
UseTagseGFPExpressionMammalianMutationdeleted amino acids 1-417PromoterCMVAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-NUbo-GFP-His6
Plasmid#212790PurposeCopper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeastDepositorInsertNUbo-GFP-His6
UseIntegrative vectorTagsExpressionYeastMutationPromoterCUP1Available sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET19-TdTwt
Plasmid#207466PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT)DepositorInsertTdTwt (Dntt Mouse)
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-SOX4
Plasmid#110360PurposeMammalian expression of FLAG-tagged SOX4DepositorInsertSOX4 (SOX4 Human)
UseTagsFLAGExpressionMammalianMutationlacking 5' UTRPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SpCas9-2A-GFP-noITR
Plasmid#118415PurposeCas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expressionDepositorInsertCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTV-Sox2_5prime-1000-24MS96T-3F_NeoR
Plasmid#174884PurposeTargeting vector for generating Sox2 STREAMING-tag KI cellDepositorInsertSTREAMING-tag with homology arms for Sox2
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-Flpo
Plasmid#183535PurposeINTRSECTDepositorInsertFlpo
UseAAVTagsExpressionMutationNonePromoterCaMKIIaAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-L713F
Plasmid#174794PurposeBacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)DepositorInsertPARP1-L713F (PARP1 Human)
UseTags6xHisExpressionBacterialMutationL713F, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
MARIO/ pcDNA3
Plasmid#124838Purposemammalian expression of MARIO, a genetically-encoded fluorescent Mg2+ sensorDepositorInsertMARIO
UseTagsNo tagExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
miniCGBE1 (pBM1219)
Plasmid#140253PurposeCMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFPDepositorInsertBE4max(R33A)-ΔUGI
UseTagsExpressionMammalianMutationR33A in rAPOBEC1, D10A in Cas9PromoterCMVAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSKΔBS-SD_MCS_SA(NBSI)-24xMS2-sirius-96xopto-TetO-Int-3xFLAG-NeoR-GS4
Plasmid#177265PurposeSTREAMING-tag cassetteDepositorInsertSTREAMING-tag cassette
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only