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Plasmid#114676PurposeThis construct was used to express mouse Otop3 in Xenopus laevis oocytesDepositorInsertOtopetrin 3 (Otop3 Mouse)
UseFor generating the mrna, which can highly express…TagsExpressionMutationPromoterT7Available sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
3789 pmko-neo RNAi-GFP
Plasmid#8881DepositorInsertGFP
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFVp-CRISPRoffv2.1-IRES-BlastR
Plasmid#207181PurposeLentiviral Expression Plasmid for CRISPRoff2.1 driven by SFFV PromoterDepositorInsertCRISPRoffv2.1
UseCRISPR and LentiviralTagsHA and TagBFPExpressionMammalianMutationPromoterSFFVpAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC-JAG1-mVenus
Plasmid#171170PurposeRetroviral vector for CMV promoter driven expression of JAG1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).DepositorInsertJAG1 (JAG1 Human)
UseRetroviralTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBFC1171
Plasmid#231994PurposeCRISPRi-ART crRNA only plasmid encoding crystal violet-inducible crRNA (for dRfxCas13d) with 2xBsaI spacer cloning siteDepositorInsertcrRNA of dRfxCas13d
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpJEx (Jungle Express)Available sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBFC0984
Plasmid#231992PurposeCRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning siteDepositorInsertsdRfxCas13d
crRNA with 2xBsaI spacer
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and pTetAvailable sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBFC0993
Plasmid#231993PurposeCRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacerDepositorInsertsdRfxCas13d
crRNA with negative control (RFP-targeting) spacer
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and pTetAvailable sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0-MRLC-DD-GFP
Plasmid#187260PurposeLentiviral expression of myosin regulatory light chain-DD mutantDepositorInsertMRLC1 (MYL9 Human)
UseLentiviralTagsEGFPExpressionMutationT18D, S19DPromoter5' LTRAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0-MRLC-AA-GFP
Plasmid#187259PurposeLentiviral expression of myosin regulatory light chain-AA mutantDepositorInsertMRLC1 (MYL9 Human)
UseLentiviralTagsEGFPExpressionMutationT18A,S19APromoter5' LTRAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-TJP1
Plasmid#227300PurposeDonor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)DepositorInsertTJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only