Addgene

We narrowed to 13,541 results for: pET

Showing: 8241 - 8260 of 13541 results

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pmiRGLO-GRM4-202

Plasmid

#117347

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR GRM4-202

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Murine PGK-1

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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Zebrafish-gRNA-0003

Plasmid

#42243

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

gRNA-fh site #1 (fh Zebrafish)

Use

CRISPR; Zebrafish expression

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only
pMAL c2x Tral FL

Plasmid

#113012

Purpose

For bacterial expression of MBP fusion of full-length Drosophila Tral WT

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length tral (tral Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hmga2 ATG m7

Plasmid

#52035

Purpose

encodes mutant HMGA2

Depositor

Julian Downward

Article

Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

Insert

Hmga2 (Hmga2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

all seven let-7 sites in the 3′ UTR mutated (‘m7’)

Promoter

CMV

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pInducer20-iCreb5(HA)

Plasmid

#195023

Purpose

HA-tagged Creb5 in pInducer20 lentivirus destination vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa) (CREB5 Bovine)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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GAL4-Creb5 (1- 128)

Plasmid

#195031

Purpose

GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa)

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa) (CREB5 Bovine)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K48R)-GFP

Plasmid

#212793

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K48R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K48R)-GFP

Use

Integrative vector

Tags

Expression

Yeast

Mutation

CUb(K48R)

Promoter

CUP1

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K63R)-GFP

Plasmid

#212794

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K63R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K63R)-GFP

Use

Integrative vector

Tags

Expression

Yeast

Mutation

CUb(K63R)

Promoter

CUP1

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-NUbo-E3(48) aa24-150-VSV

Plasmid

#212747

Purpose

Constitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48) aa24-150

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Cue1 ΔU7BD

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-myc-E3(1) C885A-CUbo-VSV

Plasmid

#212738

Purpose

Consitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-NLS-VSV and myc

Expression

Yeast

Mutation

HOIP(C885A)

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

Plasmid

#212805

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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H6GB1tevP53(304-393)

Plasmid

#200313

Purpose

bacterial expression of p53 TET-CTD

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53(304-393) (TP53 Human)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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H6GB1tevP53(363-393)

Plasmid

#200314

Purpose

bacterial expression of P53 CTD

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53(363-393) (TP53 Human)

Use

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmeIF4G_617-675-K633PQ638P-GB1_AD

Plasmid

#148510

Purpose

Bacterial Expression of DmeIF4G_617-675-K633PQ638P

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_617-675-K633PQ638P (eIF4G1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmeIF4Ga_617-630-DmThor_64-83-GB1_AD

Plasmid

#148513

Purpose

Bacterial Expression of DmeIF4Ga_617-630-DmThor_64-83

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4Ga_617-630-DmThor_64-83 (eIF4G1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-83-Y54AM59A-GB1_AA

Plasmid

#148284

Purpose

Bacterial Expression of DmThor_50-83-Y54AM59A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor_50-83-Y54AM59A (Thor Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-DmThor_Z

Plasmid

#148188

Purpose

Bacterial Expression of DmThor

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor (Thor Fly)

Use

Tags

Expression

Bacterial

Mutation

two silent mutations compared to the sequence giv…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-DmThor-CLL78AAA_Z

Plasmid

#148189

Purpose

Bacterial Expression of DmThor-CLL78AAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-CLL78AAA (Thor Fly)

Use

Tags

Expression

Bacterial

Mutation

two silent mutations compared to the sequence giv…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-DmThor-GB1_Z

Plasmid

#148192

Purpose

Bacterial Expression of DmThor

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor (Thor Fly)

Use

Tags

Expression

Bacterial

Mutation

two silent mutations compared to the sequence giv…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-DmThor-Y54AM59A_Z

Plasmid

#148193

Purpose

Bacterial Expression of DmThor-Y54AM59A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-Y54AM59A (Thor Fly)

Use

Tags

Expression

Bacterial

Mutation

two silent mutations compared to the sequence giv…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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