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Plasmid#148264PurposeBacterial Expression of DmCup_325-376-R329ER336EDepositorInsertDmCup_325-376-R329ER336E (cup Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmCUP_325-376-R329ER336ER375E-GB1_AA
Plasmid#148265PurposeBacterial Expression of DmCup_325-376-R329ER336ER375EDepositorInsertDmCup_325-376-R329ER336ER375E (cup Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmCUP_325-376-R336E-GB1_AA
Plasmid#148266PurposeBacterial Expression of DmCup_325-376-R336EDepositorInsertDmCup_325-376-R336E (cup Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPS1-Venus
Plasmid#133118PurposePlasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pYPS1 driving a Venus.DepositorInsertpYPS1-YFP
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMK2-Venus
Plasmid#133119PurposePlasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pCMK2 driving a Venus.DepositorInsertpCMK2-YFP
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGYP7-Venus
Plasmid#133120PurposePlasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pGYP7 driving a Venus.DepositorInsertpGYP7-YFP
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA
Plasmid#140223PurposeConstitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activatorDepositorInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein
UseTagsExpressionMammalianMutationD832APromoterPGKAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
EED-F97A
Plasmid#131326PurposegRNA to generate F97A cage-mutant EEDDepositorInsertEED-KO-gRNA-2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSI-check2-TAB2-3'UTR
Plasmid#97157PurposeFor dual luciferase report assayDepositorInsertTAB2 3'UTR (TAB2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLENTI-CMV-GFP-TREX1 delta C (aa1-235) BLAST
Plasmid#164229Purposelentiviral plasmid for GFP-hTREX1 delta C (aa1-235)DepositorInsertTREX1 (TREX1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationdeleted amino acids 235-314PromoterCMVAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLENTI CMV GFP-TREX1 delta N (aa236-314) BLAST
Plasmid#164235Purposelentiviral plasmid for GFP-TREX1 delta N (aa236-314)DepositorInsertTREX1 (TREX1 Human)
UseLentiviralTagsGFPExpressionMammalianMutationdeletion of amino acids 1-234PromoterCMVAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
myristoylated ct-GRK2
Plasmid#178734PurposeN-myristoylation signaling peptide of Src and the C-terminal fragment of G-protein coupled receptor kinase 2 (ct-GRK2)DepositorInsertGRK2 (GRK2 Bovine)
UseLentiviralTagsSrc 1-15ExpressionMutationonly amino acids 548-671PromoterUbiquitinAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
MGAT1-GFP
Plasmid#163647PurposeExpresses GFP tagged MGAT1 in mammalian cellsDepositorInsertalpha-1,3-mannosyl-glycoprotein beta-1, 2-N-acetylglucosaminyltransferase (MGAT1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mEGFP-Map4
Plasmid#105571PurposeT7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNADepositorInsertMAP4 (Map4 Mouse)
UseIn vitro transcriptionTagsmEGFPExpressionMutationPromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
HP21-TPI-WT-no_introns
Plasmid#131044PurposeExpresses TPI-Wildtype NMD reporter without introns and with a 21 nt hairpin in 5'UTRDepositorInsertTPI-Wildtype-no introns (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_gRNA1TERTKO_BsagRNA5tertko_2A-GFP
Plasmid#198863PurposePlasmid encoding for 2 gRNAs targeting the human TERT geneDepositorInsertTERT sgRNA (TERT Human)
UseTagsExpressionMammalianMutationPromoteru6Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas9-CAT
Plasmid#80323PurposeEncodes Cas9 and chloramphenicol acetyltransferase (CAT)DepositorInsertsCas9
Chloramphenicol acetyltransferase
UseCRISPRTags3X FLAG and NLSExpressionMutationPromoterTgTUB1Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs2-RAC1 G12V
Plasmid#86139PurposeRetroviral vector expressing RAC1(G12V)-IRES-Bsd-p2A-RFPDepositorInsertRAC1 (RAC1 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationG12VPromoterLTRAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs3-NRAS G13D
Plasmid#86144PurposeRetroviral vector expressing NRAS(G13D)-IRES-RFPDepositorInsertNRAS G13D (NRAS Human)
UseRetroviralTagsExpressionMammalianMutationG13DPromoterLTRAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry-Cenexin
Plasmid#186435PurposeExpresses mCherry Cenexin in mammalian cells and zebrafish embryosDepositorInsertCenexin (ODF2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only