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Addgene
Showing: 8221 - 8240 of 16034 results

  1. pBTRCk-pVhb-maACR

    Plasmid
    #215651
    Purpose
    pBBR1 Origin, KanR, with maACR gene under Vhb promoter
    Depositor
    Brian Pfleger
    Insert
    acyl-CoA reductase
    Use
    Tags
    Maltose binding protein and His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Vhb
    Availability
    Academic Institutions and Nonprofits only
    215651-plasmid-map-sequence-id-440374

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  7. GRLF1

    Plasmid
    #36195
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GRLF1 (Codon Devices Synthesized) (ARHGAP35 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    Contains the Ras homolog domain only (amino acid …
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. AICSDP-63: ACTN2-mEGFP

    Plasmid
    #124607
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Insert
    ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    124607-plasmid-map-sequence-id-244336

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  9. p663-UBC-miniTurbo-V5-CDK19_IDG-K

    Plasmid
    #158175
    Purpose
    Gateway destination clone of CDK19 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDK19 (CDK19 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    158175-plasmid-map-sequence-id-311145

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  10. p663-UBC-miniTurbo-V5-DSTYK _IDG-K

    Plasmid
    #158182
    Purpose
    Gateway destination clone of DSTYK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-DSTYK (DSTYK Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    158182-plasmid-map-sequence-id-314617

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  11. p667-UBC-DYRK3-V5-miniTurbo_IDG-K

    Plasmid
    #158190
    Purpose
    Gateway destination clone of DYRK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    DYRK3-V5-miniTurbo (DYRK3 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    R293H- please see depositor comments
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    158190-plasmid-map-sequence-id-328919

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  12. p667-UBC-HIPK3-V5-miniTurbo_IDG-K

    Plasmid
    #158191
    Purpose
    Gateway destination clone of HIPK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    HIPK3-V5-miniTurbo (HIPK3 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    158191-plasmid-map-sequence-id-310258

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  13. p663-UBC-miniTurbo-V5-CDK7_IDG-K

    Plasmid
    #158194
    Purpose
    Gateway destination clone of CDK7 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDK7 (CDK7 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    158194-plasmid-map-sequence-id-310269

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  14. p663-UBC-miniTurbo-V5-CDK13_IDG-K

    Plasmid
    #135266
    Purpose
    Gateway destination clone of CDK13 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDK13 (CDK13 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    135266-plasmid-map-sequence-id-268080

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  15. p663-UBC-TurboID-V5-CDK13_IDG-K

    Plasmid
    #135267
    Purpose
    Gateway destination clone of CDK13 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TurboID-V5-CDK13 (CDK13 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    135267-plasmid-map-sequence-id-269144

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  16. pSBtet-2xMARS-nGFP-puro

    Plasmid
    #205239
    Purpose
    DOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon system
    Depositor
    Jeremy Baskin
    Insert
    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    Use
    Transposon
    Tags
    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    tight TRE promoter
    Availability
    Academic Institutions and Nonprofits only
    205239-plasmid-map-sequence-id-404740

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  19. p663-UBC-miniTurbo-V5-CDKL5_IDG-K

    Plasmid
    #170578
    Purpose
    Gateway destination clone of CDKL5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDKL5 (CDKL5 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    E854G- please see depositor comments
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    170578-plasmid-map-sequence-id-340993

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  20. p663-UBC-miniTurbo-V5-LMTK2_IDG-K

    Plasmid
    #170580
    Purpose
    Gateway destination clone of LMTK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-LMTK2 (LMTK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    170580-plasmid-map-sequence-id-341636

    Enlarge

Showing: 8221 - 8240 of 16034 results