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pU6-SAG1-DHFR

Plasmid

#80322

Purpose

Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

SAG1 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA

Plasmid

#140222

Purpose

Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR

Use

Tags

mCherry

Expression

Mammalian

Mutation

D832A

Promoter

PGK

Available since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-DHFR

Plasmid

#80329

Purpose

Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

DHFR-TSc3

Use

CRISPR

Tags

Expression

Mutation

Promoter

DHFR-TS 5' UTR

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pAEMXT-eLACCO1.1

Plasmid

#167946

Purpose

Fluorescent biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

CD59-eLACCO1.1-GPI

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pHis2-Arf1 (17-181)-Q71L

Plasmid

#184808

Purpose

Bacterial expression of Arf1 (aa17-181, Q71L mutation)

Depositor

James Hurley

Article

Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

Insert

Arf1 (ARF1 Human)

Use

Tags

His6

Expression

Bacterial

Mutation

aa 17-181 only, Q71L

Promoter

T7

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgUBA5

Plasmid

#86132

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting UBA5

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA targeting UBA5 (UBA5 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 15, 2017

Availability

Academic Institutions and Nonprofits only

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pAAP2

Plasmid

#73211

Purpose

express Assembly-activating protein (AAP) of adeno-associated virus serotype 2 (AAV2)

Depositor

Luk Vandenberghe

Article

Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

Insert

AAP2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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SRSF8_RRM_pGEX

Plasmid

#135119

Purpose

Recombinant expression of SRSF8 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

SRSF8 (SRSF8 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Decoy

Plasmid

#80324

Purpose

Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

Decoy sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pAEMXT-deLACCO

Plasmid

#167947

Purpose

Fluorescent biosensor control for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

CD59-deLACCO-GPI

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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SRSF9_2xRRM_pGEX

Plasmid

#135120

Purpose

Recombinant expression of SRSF9 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

SRSF9 (SRSF9 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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HNRNPCL1_RRM_pGEX

Plasmid

#135116

Purpose

Recombinant expression of HNRNPCL1 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

HNRNPCL1 (HNRNPCL1 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Nov. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs2-RAC1

Plasmid

#86138

Purpose

Retrolviral vector expressing RAC1-IRES-Bsd-p2A-RFP

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

RAC1 (RAC1 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only
RBM15B_3xRRM_pGEX

Plasmid

#135125

Purpose

Recombinant expression of RBM15B with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM15B (RBM15B Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-TTF-1-gRNA

Plasmid

#101857

Purpose

Introducing a gRNA targeting human TTF-1 (NKX2-1) into cells

Depositor

David Mu

Article

Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

Insert

gRNA targeting TTF-1 (NKX2-1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLN410 (FuGW-S(E2F1)p-ECFP sponge control)

Plasmid

#105182

Purpose

Module 2 control - synthetic promoter E2F1 drives ECFP only (no miR1 binding sites)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(E2F1)p-ECFP (Cerulean) sponge control

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-RAP2A-GFP

Plasmid

#86124

Purpose

Retroviral vector expressing RAP2A-GFP-IRES-Bsd

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

RAP2A (RAP2A Human)

Use

Retroviral

Tags

3xFLAG and GFP

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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RBMS3_2xRRM_pGEX

Plasmid

#135142

Purpose

Recombinant expression of RBMS3 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBMS3 (RBMS3 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 10, 2021

Availability

Academic Institutions and Nonprofits only

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RBM24_RRM_pGEX

Plasmid

#135148

Purpose

Recombinant expression of RBM24 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

RBM24 (RBM24 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmCUP_325-376-R329E-GB1_AA

Plasmid

#148263

Purpose

Bacterial Expression of DmCup_325-376-R329E

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmCup_325-376-R329E (cup Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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