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Plasmid#203224PurposeFor protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertDNPH1 (DNPH1 Human)
UseTagsHis tag (with TEV cleavage site)ExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTAL_RFP
Plasmid#43859DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
hEBI3 pEF6-V5 (MJS_002)
Plasmid#72490PurposeExpression of human EBI3 in mammalian cells (V5 tag)DepositorInsertEBI3 (EBI3 Human)
UseTagsHis/V5ExpressionMammalianMutationPromoterEF-1 alphaAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pTAL_GFP
Plasmid#43858DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
hPlekhm1-dsRedM
Plasmid#73592PurposeExpress human Plekhm1 in mammalian cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTagsdsRedExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-SERINC2-202
Plasmid#117364PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR SERINC2-202
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-iLMO4 (GLucM23-iChloC-EYFP)
Plasmid#114102Purposefusion protein of Gaussia luciferase variant M23, improved slowChloC anion conducting ChR, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertsGaussia luciferase, M23
improved slowChloC artificial anion conducting ChR
Enhanced Yellow Fluorescent Protein
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_-CMV-Pcdh15_mini_V4-CD2
Plasmid#199184PurposeAn AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.DepositorInsertmmPcdh15_mini_V4-CD2
UseAAVTagsExpressionMutationEC4, EC5, EC6, EC7, EC8 are deleted from msPCDH15…PromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-Pcdh15_mini_V7-CD2
Plasmid#199186PurposeAn AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.DepositorInsertmmPcdh15_mini_V7-CD2
UseAAVTagsExpressionMammalianMutationEC4, EC5, EC6, EC9, EC10 are deleted from msPCDH1…PromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-Pcdh15_mini_V8-CD2
Plasmid#199187PurposeAn AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.DepositorInsertmmPcdh15_mini_V8-CD2
UseAAVTagsExpressionMutationEC5, EC6, EC8, EC9, EC10 are deleted from msPCDH1…PromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVenus-CyclinB1(R42A) (1317)
Plasmid#39873DepositorInsertCyclinB1(R42A) (CCNB1 Human)
UseTagsVenusExpressionMammalianMutationR42A = destruction box mutations = non-destructib…PromoterAvailable sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mqgDUX4mqg*
Plasmid#21177DepositorInsertDUX4 (DUX4 Human)
UseTagsExpressionBacterial and MammalianMutationPoint mutation at nucleotide 6163 (numbering acco…PromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-BAX-201
Plasmid#117332PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR BAX-201
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-Neo-PTPRT (R632X)
Plasmid#16629DepositorInsertPTPRT R632X (PTPRT Human)
UseTagsExpressionMammalianMutationtruncated R632X mutantPromoterAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-sbGLuc-CheRiff-EGFP
Plasmid#114108Purposefusion protein of Gaussia luciferase variant slow burn, synthetic channelrhodopsin CheRiff, and enhanced green fluorescent protein for bioluminescent optogeneticsDepositorInsertsGaussia luciferase, slow burn
CheRiff, synthetic ChR
Enhanced Green Fluorescent Protein
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0004
Plasmid#42244DepositorInsertgRNA-fh site #2 (fh Zebrafish)
UseCRISPR; Zebrafish expressionTagsExpressionMutationPromoterT7Available sinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCCL-PGK-SPdCas9-BFP-EGFP
Plasmid#66821PurposedCas9 fused to BFP and EGFPDepositorInsertdSpCas9-BFP-EGFP
UseTagsdSpCas9-BFP-EGFPExpressionMammalianMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEcRNAP5
Plasmid#128939PurposepET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, ZDepositorInsertsUseTagsHisExpressionBacterialMutationResidues 234-241 are substituted for a PreScissio…PromoterT7Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJT131_GalL_cCDA1-NGBE3
Plasmid#145095PurposeExpressing base editor cCDA1-NGBE3 in yeast cellsDepositorInsertcCDA1-NGBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only