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Plasmid#16577DepositorInsertHomo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgMyc_1
Plasmid#138324PurposeExpress guide RNA 2 for mouse MycDepositorInsertcMyc sgRNA (Myc Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
TIRAP-TAP_IRES_GFP
Plasmid#52740PurposeRetroviral vector expresses hTIRAP-3xHA and a target site for the biotin ligase BirA upstream of IRES and GFPDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTags3x HA, 3x TEV, and BirA target siteExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry)
Plasmid#216531PurposeTargeting vector for CRISPRd generationDepositorInsertABI-dCas9-mCherry
UseCRISPRTagsmCherryExpressionMammalianMutationPromoterTRE3GAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
plRL2
Plasmid#163631PurposeOptimized M. tuberculosis CRISPRi plasmid; L5 integrating.DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterTet-OnAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
plRL117
Plasmid#163635PurposeOptimized M. smegmatis CRISPRi plasmid; L5 integrating.DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterTet-OnAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-eLACCO1.1
Plasmid#167944PurposeFluorescent biosensor for extracellular L-lactateDepositorInserteLACCO1.1
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
plRL118
Plasmid#163632PurposeRenilla luciferase reporter plasmid; Giles integrating.DepositorTypeEmpty backboneUseLuciferaseTagsExpressionBacterialMutationPromoterMycobacterial Optimized Promoter (MOP)Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLN397 (FuGW-S(E2F1)p-Sponge7)
Plasmid#105181PurposeModule 2 - synthetic promoter E2F1 drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)DepositorInsertS(E2F1)p-Sponge7
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLN553 (FuGW-S(MAFK)p-Sponge7)
Plasmid#105203PurposeModule 2 - synthetic promoter MAFK drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)DepositorInsertS(MAFK)p-Sponge7
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eBE-S1
Plasmid#101092PurposeExpresses eBE-S1 in mammalian cellsDepositorInserteBE-S1
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-deLACCO
Plasmid#167945PurposeFluorescent biosensor control for extracellular L-lactateDepositorInsertdeLACCO
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti_stgRNA-Puro
Plasmid#164124PurposeBackbone for lentiviral stgRNA library construction (library 1 and 2)DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhuman U6Available sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-tetO-NGN2
Plasmid#197090PurposePiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiationDepositorInsertNGN2 (NEUROG2 Human)
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
psigma2-EGFP
Plasmid#53610PurposeExpresses sigma2 of adaptor protein of AP2DepositorInsertsigma2 (Ap2s1 Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSCVpic2neo-hTERT-FF2
Plasmid#164910PurposeExpresses hTERT cDNA and FF2 shRNA in mammalian cellsDepositorInsertsUseRetroviralTagsHA tagExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCGN-HCF-1-N1011Δ382-450
Plasmid#92322PurposeN-term of HCF-1 protein without amino acids 382-450DepositorInsertHCF-1 (HCFC1 Human)
UseTagsHAExpressionMammalianMutationAA 2-1011 with internal deletion Δ382-450PromoterCMVAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd
Plasmid#216171PurposeExpress the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experimentDepositorInsertsgRNA- PRDM1.bs- ZEB2.locus (PRDM1 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
CB6-GFP-TAOK1 (K57A)
Plasmid#197109PurposeExpression of kinase-deficient GFP-tagged TAOK1 (K57A) / PSK2 (K57A) in mammalian cellsDepositorInsertTAOK1 (K57A) (TAOK1 Human)
UseTagsGFPExpressionMammalianMutationChanged K 57 to APromoterCMVAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABE-neo-hTERT
Plasmid#1774PurposeRetroviral expression of hTERT. Used to create immortalized cell lines.DepositorInserthTERT (TERT Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only